ArticleMolecular and cellular endocrinology2014
Hypo-glycosylated human follicle-stimulating hormone (hFSH(21/18)) is much more active in vitro than fully-glycosylated hFSH (hFSH(24)).
Article in Molecular and cellular endocrinology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to trial NCT03868202 (Follicle Stimulating Hormone), which is not on this map. Cited by 42 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Follicle Stimulating Hormone (FSH) Glycosylation in Women: Effect of Estradiol
Who cites it
42 citing papers in PubMed, 71 citations in OpenAlex.
- Urinary and Recombinant Follicle-Stimulating Hormone in Supporting Primate Follicular Development In Vitro.Current issues in molecular biology · 2026Article
- Gonadotropin trajectories among postmenopausal women not using hormone therapy.Menopause (New York, N.Y.) · 2026Article
- The N-Linked Glycosylation Asn191 and Asn199 Sites Are Controlled Differently Between PKA Signal Transduction and pEKR1/2 Activity in Equine Follicle-Stimulating Hormone Receptor.Current issues in molecular biology · 2025Article
- Article
- Oocyte quality is enhanced by hypoglycosylated FSH through increased cell-to-cell interaction during mouse follicle development.Development (Cambridge, England) · 2023Article
- Differential effects of follicle-stimulating hormone glycoforms on the transcriptome profile of cultured rat granulosa cells as disclosed by RNA-seq.bioRxiv : the preprint server for biology · 2023Article
- Follicle-Stimulating Hormone Glycosylation Variants Distinctly Modulate Pre-antral Follicle Growth and Survival.Endocrinology · 2022Article
- Determination of Half-lives of Circulating FSH and LH Glycoforms in Women During GnRH Receptor Blockade.The Journal of clinical endocrinology and metabolism · 2022Article
- Inhibin Inactivation in Female Mice Leads to Elevated FSH Levels, Ovarian Overstimulation, and Pregnancy Loss.Endocrinology · 2022Article
- Human FSH Glycoform α-Subunit AsparagineFrontiers in endocrinology · 2022Article
- Low- and Fully N-Glycosylated Gonadotropins Circulating in Women With Polycystic Ovary Syndrome.Journal of the Endocrine Society · 2021Article
- Hypo-glycosylated hFSH drives ovarian follicular development more efficiently than fully-glycosylated hFSH: enhanced transcription and PI3K and MAPK signaling.Human reproduction (Oxford, England) · 2021Article
- Identification and Relative Quantification of hFSH Glycoforms in Women's Sera via MS-PRM-Based Approach.Pharmaceutics · 2021Article
- A Novel Follitropin Analog Inhibits Follitropin Activity In Vitro.Pharmaceutics · 2021Article
- Differential FSH Glycosylation Modulates FSHR Oligomerization and Subsequent cAMP Signaling.Frontiers in endocrinology · 2021Article
- Recent advances in understanding gonadotropin signaling.Faculty reviews · 2021Review
- New Human Follitropin Preparations: How Glycan Structural Differences May Affect Biochemical and Biological Function and Clinical Effect.Frontiers in endocrinology · 2021Review
- Gonadotropin Glycoforms Circulating in Women Using Progestins of the Levonorgestrel Family for Contraception.Journal of the Endocrine Society · 2020Article
- In vitro ovarian follicle growth: a comprehensive analysis of key protocol variables†.Biology of reproduction · 2020Review
- Bioactivity of recombinant hFSH glycosylation variants in primary cultures of porcine granulosa cells.Molecular and cellular endocrinology · 2020Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
Abstract
Hypo-glycosylated hFSH(21/18) (possesses FSHβ(21) and FSHβ(18)bands) was isolated from hLH preparations by immunoaffinity chromatography followed by gel filtration. Fully-glycosylated hFSH(24) was prepared by combining the fully-glycosylated FSHβ(24) variant with hCGα and isolating the heterodimer. The hFSH(21/18) glycoform preparation was significantly smaller than the hFSH(24) preparation and possessed 60% oligomannose glycans, which is unusual for hFSH. Hypo-glycosylated hFSH(21/18) was 9- to 26-fold more active than fully-glycosylated hFSH(24) in FSH radioligand assays. Significantly greater binding of (125)I-hFSH(21/18) tracer than hFSH(24) tracer was observed in all competitive binding assays. In addition, higher binding of hFSH(21/18) was noted in association and saturation binding assays, in which twice as much hFSH(21/18) was bound as hFSH(24). This suggests that more ligand binding sites are available to hFSH(21/18) in FSHR than to hFSH(24). Hypo-glycosylated hFSH(21/18) also bound rat FSHRs more rapidly, exhibiting almost no lag in binding, whereas hFSH(24) specific binding proceeded very slowly for almost the first hour of incubation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.