Evidence map›Paper›PMID 24290809›Full record

ArticleBiomaterials2014

Photopatternable and photoactive hydrogel for on-demand generation of hydrogen peroxide in cell culture.

Shaun P Garland, Royal Y Wang, Vijay Krishna Raghunathan, Kit S Lam, Christopher J Murphy, Paul Russell, Gang Sun, Tingrui Pan

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biomaterials, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Shaun P GarlandMicro-Nano Innovations (MiNI) Laboratory, Department of Biomedical Engineering, University of California Davis, Davis, CA 95616, USA.
Royal Y WangMicro-Nano Innovations (MiNI) Laboratory, Department of Biomedical Engineering, University of California Davis, Davis, CA 95616, USA.
Vijay Krishna RaghunathanDepartment of Surgical & Radiological Sciences, School of Veterinary Medicine, University of California Davis, Davis, CA 95616, USA.
Kit S LamDepartment of Biochemistry and Molecular Medicine, University of California Davis, Davis, CA 95616, USA.
Christopher J MurphyDepartment of Surgical & Radiological Sciences, School of Veterinary Medicine, University of California Davis, Davis, CA 95616, USA; Department of Ophthalmology & Vision Sciences, School of Medicine, University of California Davis, Davis, CA 95616, USA.
Paul RussellDepartment of Surgical & Radiological Sciences, School of Veterinary Medicine, University of California Davis, Davis, CA 95616, USA.
Gang SunDivision of Textiles and Clothing, University of California Davis, Davis, CA 95616, USA.
Tingrui PanMicro-Nano Innovations (MiNI) Laboratory, Department of Biomedical Engineering, University of California Davis, Davis, CA 95616, USA. Electronic address: tingrui@ucdavis.edu.

Funding

VISION RESEARCH CORE GRANTP30EY012576 · NEI · UNIVERSITY OF CALIFORNIA DAVIS · PI LISA M OAKES · 1999 to 2026
$18.9M
Vision Science Training Program: Cornea to CortexT32EY015387 · NEI · UNIVERSITY OF CALIFORNIA DAVIS · PI MARIE E BURNS · 2003 to 2026
$4.8M
Biophysical Cues and Corneal Wound HealingR01EY019970 · NEI · UNIVERSITY OF CALIFORNIA AT DAVIS · PI MURPHY, CHRISTOPHER JOHN · 2011 to 2020
$3.5M
Role of Biophysical Cues in the Pathogenesis of GlaucomaR01EY019475 · NEI · UNIVERSITY OF CALIFORNIA AT DAVIS · PI RUSSELL, PAUL · 2010 to 2013
$1.5M
NEI NIH HHS P30 EY012576NEI NIH HHS P30EY12576NEI NIH HHS R01 EY019475NEI NIH HHS R01EY019475NEI NIH HHS R01 EY019970NEI NIH HHS R01EY019970NEI NIH HHS T32 EY015387NEI NIH HHS T32EY15387-9
6 · The paper itself

Abstract

Oxidative stress, largely mediated by reactive oxygen species (ROS), is a nearly ubiquitous component in complex biological processes such as aging and disease. Optimal in vitro methods used in elucidating disease mechanisms would deliver of low levels of hydrogen peroxide, emulating the in vivo pathological state, but current methods are limited by kinetic stability or accurate measurement of the dose administered. Here we present an in vitro platform that exploits anthraquinone catalysts for the photocatalytic production of hydrogen peroxide. This system can be dynamically tuned to provide constant generation of hydrogen peroxide at a desired physiologic rate over at least 14 days and is described using a kinetic model. Material characterization and stability is discussed along with a proof-of-concept in vitro study that assessed the viability of cells as they were oxidatively challenged over 24 h at different ROS generation rates.

Indexed as

HydrogelsPhotochemical ProcessesCell LineHydrogen PeroxideKineticsModels, BiologicalOxidative StressHydrogelsHydrogen PeroxideAnthraquinoneHydrogen peroxideOxidative stressROS

Identifiers

PMID24290809
PMCPMC3992930

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.