ArticleCell reports2013
Integrin-matrix clusters form podosome-like adhesions in the absence of traction forces.
Article in Cell reports, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 68 papers.
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Who cites it
68 citing papers in PubMed.
- Vimentin promotes collective cell migration through collagen networks via increased matrix remodeling and spheroid fluidity.Communications biology · 2026Article
- Bifunctional Surfaces With Immobilized Antibodies and Bioactive Peptides Mediate Selective Capture and Proliferation of Endothelial Colony-Forming Cells.Advanced healthcare materials · 2026Article
- Super-Resolution Axial Imaging for Quantifying Piconewton Traction Forces in Live Cells.Angewandte Chemie (International ed. in English) · 2025Article
- PI(3,4,5)P3-mediated Cdc42 activation regulates macrophage podosome assembly.Cellular and molecular life sciences : CMLS · 2025Article
- Cell adhesion and spreading on fluid membranes through microtubules-dependent mechanotransduction.Nature communications · 2025Article
- N-cadherin crosstalk with integrin weakens the molecular clutch in response to surface viscosity.Nature communications · 2024Article
- Morphodynamics of T-lymphocytes: Scanning to spreading.Biophysical journal · 2024Review
- Osteoclasts at Bone Remodeling: Order from Order.Results and problems in cell differentiation · 2024Review
- Mesenchymal Migration on Adhesive-Nonadhesive Alternate Surfaces in Macrophages.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2023Article
- Microglia degrade Tau oligomers deposit via purinergic P2Y12-associated podosome and filopodia formation and induce chemotaxis.Cell & bioscience · 2023Article
- Expression of non-phosphorylatable S5A-L-plastin exerts phenotypes distinct from L-plastin deficiency during podosome formation and phagocytosis.Frontiers in cell and developmental biology · 2023Article
- Effects of β2 Integrins on Osteoclasts, Macrophages, Chondrocytes, and Synovial Fibroblasts in Osteoarthritis.Biomolecules · 2022Review
- Pressure and stiffness sensing together regulate vascular smooth muscle cell phenotype switching.Science advances · 2022Article
- Advances in Cell-Conductive Polymer Biointerfaces and Role of the Plasma Membrane.Chemical reviews · 2022Article
- Single-Molecule Force Imaging Reveals That Podosome Formation Requires No Extracellular Integrin-Ligand Tensions or Interactions.ACS nano · 2022Article
- WASp triggers mechanosensitive actin patches to facilitate immune cell migration in dense tissues.Developmental cell · 2022Article
- StarD13 negatively regulates invadopodia formation and invasion in high-grade serous (HGS) ovarian adenocarcinoma cells by inhibiting Cdc42.European journal of cell biology · 2022Article
- Review
- Substrate Resistance to Traction Forces Controls Fibroblast Polarization.Biophysical journal · 2020Article
- Photo-Cleavable Peptide-Poly(Ethylene Glycol) Conjugate Surfaces for Light-Guided Control of Cell Adhesion.Micromachines · 2020Article
8 more citing papers are in PubMed but not listed here.
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Authors and funding
7 authors.
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Abstract
Matrix-activated integrins can form different adhesion structures. We report that nontransformed fibroblasts develop podosome-like adhesions when spread on fluid Arg-Gly-Asp peptide (RGD)-lipid surfaces, whereas they habitually form focal adhesions on rigid RGD glass surfaces. Similar to classic macrophage podosomes, the podosome-like adhesions are protrusive and characterized by doughnut-shaped RGD rings that surround characteristic core components including F-actin, N-WASP, and Arp2/Arp3. Furthermore, there are 18 podosome markers in these adhesions, though they lack matrix metalloproteinases that characterize invadopodia and podosomes of Src-transformed cells. When nontransformed cells develop force on integrin-RGD clusters by pulling RGD lipids to prefabricated rigid barriers (metal lines spaced by 1-2 μm), these podosomes fail to form and instead form focal adhesions. The formation of podosomes on fluid surfaces is mediated by local activation of phosphoinositide 3-kinase (PI3K) and the production of phosphatidylinositol-(3,4,5)-triphosphate (PIP3) in a FAK/PYK2-dependent manner. Enrichment of PIP3 precedes N-WASP activation and the recruitment of RhoA-GAP ARAP3. We propose that adhesion structures can be modulated by traction force development and that production of PIP3 stimulates podosome formation and subsequent RhoA downregulation in the absence of traction force.
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