ReviewThe Review of scientific instruments2013
Invited review article: Methods for imaging weak-phase objects in electron microscopy.
Review in The Review of scientific instruments, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
35 citing papers in PubMed.
- Crossed laser phase plates for transmission electron microscopy.Nature communications · 2026Article
- Phase and absorbance retrieval in X-ray holographic microscopy under weak illumination using physics-driven neural networks.Journal of synchrotron radiation · 2026Article
- Template Learning: Deep learning with domain randomization for particle picking in cryo-electron tomography.Nature communications · 2025Article
- Cryo-EM phase-plate images reveal unexpected levels of apparent specimen damage.bioRxiv : the preprint server for biology · 2024Article
- Article
- Cryo-electron microscopy-based drug design.Frontiers in molecular biosciences · 2024Review
- Overcoming resolution loss due to thermal magnetic field fluctuations from phase plates in transmission electron microscopy.Ultramicroscopy · 2023Article
- Bringing Structure to Cell Biology with Cryo-Electron Tomography.Annual review of biophysics · 2023Review
- Overcoming resolution loss due to thermal magnetic field fluctuations from phase plates in transmission electron microscopy.bioRxiv : the preprint server for biology · 2023Article
- Design of an ultrafast pulsed ponderomotive phase plate for cryo-electron tomography.Cell reports methods · 2023Article
- CryomicroscopyFaraday discussions · 2022Article
- Imaging biological macromolecules in thick specimens: The role of inelastic scattering in cryoEM.Ultramicroscopy · 2022Article
- Perspective: Emerging strategies for determining atomic-resolution structures of macromolecular complexes within cells.Journal of structural biology · 2022Article
- Cryo-electron tomography related radiation-damage parameters for individual-molecule 3D structure determination.Frontiers in chemistry · 2022Review
- Conquer by cryo-EM without physically dividing.Biochemical Society transactions · 2021Review
- Making the Most of your Electrons: Challenges and Opportunities in Characterizing Hybrid Interfaces with STEM.Materials today (Kidlington, England) · 2021Article
- Single-particle cryo-EM: alternative schemes to improve dose efficiency.Journal of synchrotron radiation · 2021Article
- High-power near-concentric Fabry-Perot cavity for phase contrast electron microscopy.The Review of scientific instruments · 2021Article
- Analyzing contrast in cryo-transmission electron microscopy: Comparison of electrostatic Zach phase plates and hole-free phase plates.Ultramicroscopy · 2020Article
- Low-dose phase retrieval of biological specimens using cryo-electron ptychography.Nature communications · 2020Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
1 author.
Funding
Abstract
Contrast has traditionally been produced in electron-microscopy of weak phase objects by simply defocusing the objective lens. There now is renewed interest, however, in using devices that apply a uniform quarter-wave phase shift to the scattered electrons relative to the unscattered beam, or that generate in-focus image contrast in some other way. Renewed activity in making an electron-optical equivalent of the familiar "phase-contrast" light microscope is based in part on the improved possibilities that are now available for device microfabrication. There is also a better understanding that it is important to take full advantage of contrast that can be had at low spatial frequency when imaging large, macromolecular objects. In addition, a number of conceptually new phase-plate designs have been proposed, thus increasing the number of options that are available for development. The advantages, disadvantages, and current status of each of these options is now compared and contrasted. Experimental results that are, indeed, superior to what can be accomplished with defocus-based phase contrast have been obtained recently with two different designs of phase-contrast aperture. Nevertheless, extensive work also has shown that fabrication of such devices is inconsistent, and that their working lifetime is short. The main limitation, in fact, appears to be electrostatic charging of any device that is placed into the electron diffraction pattern. The challenge in fabricating phase plates that are practical to use for routine work in electron microscopy thus may be more in the area of materials science than in the area of electron optics.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.