ArticleBMC genomics2013
Morphogenesis-regulated localization of protein kinase A to genomic sites in Candida albicans.
Article in BMC genomics, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 20 citations in OpenAlex.
- Article
- The Cdc25 and Ras1 Proteins ofJournal of fungi (Basel, Switzerland) · 2023Article
- Presenting a codon-optimized palette of fluorescent proteins for use in Candida albicans.Scientific reports · 2020Article
- The possible molecular mechanisms of farnesol on the antifungal resistance of C. albicans biofilms: the regulation of CYR1 and PDE2.BMC microbiology · 2018Article
- The 5' Untranslated Region of themSphere · 2018Article
- A Bimolecular Fluorescence Complementation Tool for Identification of Protein-Protein Interactions inG3 (Bethesda, Md.) · 2017Article
- Hgc1-Cdc28-how much does a single protein kinase do in the regulation of hyphal development in Candida albicans?Journal of microbiology (Seoul, Korea) · 2016Review
- The Regulatory Subunit of Protein Kinase A (Bcy1) inFrontiers in microbiology · 2016Article
- Ribosomal protein S6 phosphorylation is controlled by TOR and modulated by PKA in Candida albicans.Molecular microbiology · 2015Article
- A surprising role for the Sch9 protein kinase in chromosome segregation in Candida albicans.Genetics · 2015Article
- Novel mechanism coupling cyclic AMP-protein kinase A signaling and golgi trafficking via Gyp1 phosphorylation in polarized growth.Eukaryotic cell · 2014Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe human fungal pathogen Candida albicans is able to undergo morphogenesis from a yeast to a hyphal growth form. Protein kinase A (PKA) isoforms Tpk1 and Tpk2 promote hyphal growth in a signalling pathway via the transcription factor Efg1.
resultsC. albicans strains producing epitope-tagged Tpk1 or Tpk2 were used in genome-wide chromatin immunoprecipitation on chip (ChIP chip) to reveal genomic binding sites. During yeast growth, both PKA isoforms were situated primarily within ORFs but moved to promoter regions shortly after hyphal induction. Binding sequences for Tpk2 greatly exceeded Tpk1 sites and did not coincide with binding of the PKA regulatory subunit Bcy1. Consensus binding sequences for Tpk2 within ORFs included ACCAC and CAGCA motifs that appeared to bias codon usage within the binding regions. Promoter residency of Tpk2 correlated with the transcript level of the corresponding gene during hyphal morphogenesis and occurred near Efg1 binding sites, mainly on genes encoding regulators of morphogenesis.
conclusionsPKA isoforms change their genomic binding sites from ORF to promoter regions during yeast-hyphal morphogenesis. Tpk2 binds preferentially to promoters of genes encoding regulators of cellular morphogenesis.
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