ArticleBiochemistry2013
FGF-FGFR signaling mediated through glycosaminoglycans in microtiter plate and cell-based microarray platforms.
Article in Biochemistry, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
19 citing papers in PubMed, 31 citations in OpenAlex.
- Xylosyltransferase engineering to manipulate proteoglycans in mammalian cells.Nature chemical biology · 2026Article
- Regional and disease-specific glycosaminoglycan composition and function in decellularized human lung extracellular matrix.Acta biomaterialia · 2023Article
- Synthetic Heparan Sulfate Hydrogels Regulate Neurotrophic Factor Signaling and Neuronal Network Activity.ACS applied materials & interfaces · 2022Article
- Extracellular matrix dynamics: tracking in biological systems and their implications.Journal of biological engineering · 2022Review
- Stem Cell Microarrays for Assessing Growth Factor Signaling in Engineered Glycan Microenvironments.Advanced healthcare materials · 2022Article
- The effect of electrospun scaffolds on the glycosaminoglycan profile of differentiating neural stem cells.Biochimie · 2021Article
- Review
- Functional role of glycosaminoglycans in decellularized lung extracellular matrix.Acta biomaterialia · 2020Article
- Elucidating the Interactions Between Heparin/Heparan Sulfate and SARS-CoV-2-Related Proteins-An Important Strategy for Developing Novel Therapeutics for the COVID-19 Pandemic.Frontiers in molecular biosciences · 2020Review
- Glycosaminoglycans compositional analysis of Urodele axolotl (Ambystoma mexicanum) and Porcine Retina.Glycoconjugate journal · 2019Article
- Efficient Synthesis of Heparinoid Bioconjugates for Tailoring FGF2 Activity at the Stem Cell-Matrix Interface.Bioconjugate chemistry · 2019Article
- Glycosaminoglycans from fish swim bladder: isolation, structural characterization and bioactive potential.Glycoconjugate journal · 2018Article
- Fibroblast Growth Factor Signaling Mediates Pulmonary Endothelial Glycocalyx Reconstitution.American journal of respiratory cell and molecular biology · 2017Article
- Heparan Sulfate Domains Required for Fibroblast Growth Factor 1 and 2 Signaling through Fibroblast Growth Factor Receptor 1c.The Journal of biological chemistry · 2017Article
- Article
- Perspectives on Anti-Glycan Antibodies Gleaned from Development of a Community Resource Database.ACS chemical biology · 2016Review
- Glycoarray Technologies: Deciphering Interactions from Proteins to Live Cell Responses.Microarrays (Basel, Switzerland) · 2016Review
- Fibroblast growth factor-based signaling through synthetic heparan sulfate blocks copolymers studied using high cell density three-dimensional cell printing.The Journal of biological chemistry · 2014Article
- A decorin-deficient matrix affects skin chondroitin/dermatan sulfate levels and keratinocyte function.Matrix biology : journal of the International Society for Matrix Biology · 2014Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
Fibroblast growth factor (FGF) signals cell growth through its interaction with a fibroblast growth factor receptor (FGFR) and a glycosaminoglycn (GAG) coreceptor. Here, we examine the signaling of five different FGFs (1, 2, 6, 8, and 8b) through FGFR3c. A small library of GAG and GAG-derivative coreceptors are screened to understand better the structure-activity relationship of these coreceptors on signaling. Initially, data were collected in a microtiter plate well-based cell proliferation assay. In an effort to reduce reagent requirements and improve assay throughput, a cell-based microarray platform was developed. In this cell-based microarray, FGFR3c-expressing cells were printed in alginate hydrogel droplets of ∼30 nL and incubated with FGF and GAG. Heparin was the most effective GAG coreceptor for all FGFs studied. Other GAGs, such as 2-O-desulfated heparin and chondroitin sulfate B, were also effective coreceptors. Signaling by FGF 8 and FGF 8b showed the widest tolerance for coreceptor structure. Finally, this on-chip cell-based microarray provides comparable data to a microtiter plate well-based assay, demonstrating that the coreceptor assay can be converted into a high-throughput assay.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.