ArticleJournal of proteome research2014
Accurate identification of deamidated peptides in global proteomics using a quadrupole orbitrap mass spectrometer.
Article in Journal of proteome research, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 40 citations in OpenAlex.
- Characterizing citrullination by mass spectrometry-based proteomics.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2023Review
- Proteomic Profiling of Major Peanut Allergens and Their Post-Translational Modifications Affected by Roasting.Foods (Basel, Switzerland) · 2022Article
- An efficient urine peptidomics workflow identifies chemically defined dietary gluten peptides from patients with celiac disease.Nature communications · 2022Article
- A Streamlined Data Analysis Pipeline for the Identification of Sites of Citrullination.Biochemistry · 2021Article
- Identifying transglutaminase reaction products via mass spectrometry as exemplified by the MUC2 mucin - Pitfalls and traps.Analytical biochemistry · 2020Article
- Accurate Identification of Deamidation and Citrullination from Global Shotgun Proteomics Data Using a Dual-Search Delta Score Strategy.Journal of proteome research · 2020Article
- Rapid Screening of Ellagitannins in Natural Sources via Targeted Reporter Ion Triggered Tandem Mass Spectrometry.Scientific reports · 2018Article
- Monocyte adhesion to atherosclerotic matrix proteins is enhanced by Asn-Gly-Arg deamidation.Scientific reports · 2017Article
- Proteomic profiling of archaeological human bone.Royal Society open science · 2017Article
- The PeptideAtlas of the Domestic Laying Hen.Journal of proteome research · 2017Article
- N-linked glycosite profiling and use of Skyline as a platform for characterization and relative quantification of glycans in differentiating xylem of Populus trichocarpa.Analytical and bioanalytical chemistry · 2017Article
- A Quantitative Glycomics and Proteomics Combined Purification Strategy.Journal of visualized experiments : JoVE · 2016Article
- A Description of the Clinical Proteomic Tumor Analysis Consortium (CPTAC) Common Data Analysis Pipeline.Journal of proteome research · 2016Article
- Simultaneous Enrichment of Plasma Soluble and Extracellular Vesicular Glycoproteins Using Prolonged Ultracentrifugation-Electrostatic Repulsion-hydrophilic Interaction Chromatography (PUC-ERLIC) Approach.Molecular & cellular proteomics : MCP · 2015Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Deamidation of asparagine and glutamine residues is a common post-translational modification. Researchers often rely on mass spectrometric based proteomic techniques for the identification of these post-translational sites. Mass spectral analysis of deamidated peptides is complicated and often misassigned due to overlapping (13)C peak of the amidated form with the deamidated monoisotopic peak; these two peaks are only separated by 19.34 mDa. For proper assignment, it is inherently important to use a mass spectrometer with high mass measurement accuracy and high resolving power. Herein, mouse brain tissue lysate was prepared using filter-aided sample preparation (FASP) method and Stage Tip fractionation followed by analysis on a nanoLC coupled with a quadrupole orbitrap (Q-Exactive) mass spectrometer to accurately identify more than 5400 proteins. Mass spectral data was processed using MASCOT and ProteoIQ for accurate identification of peptides and proteins. MASCOT search values for precursor and MS/MS mass tolerances were investigated, and it was determined that data searched with greater than 5 ppm precursor mass tolerance resulted in the misassignment of deamidated peptides. Peptides that were identified with a mass measurement accuracy of ±5 ppm were correctly assigned.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.