ArticleNucleic acids research2014
Spatio-temporal requirements for transposable element piRNA-mediated silencing during Drosophila oogenesis.
Article in Nucleic acids research, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers, 1 of them a synthesis that pooled it.
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Who cites it
29 citing papers in PubMed, 1 synthesis or guideline pooled it, 45 citations in OpenAlex.
- Infections by Transovarially Transmitted DMelSV in Drosophila Have No Impact on Ovarian Transposable Element Transcripts but Increase Their Amounts in the Soma.Genome biology and evolution · 2021Pooled it
- Host-transposable element coexistence: a matter of resistance, tolerance and trade-off.The EMBO journal · 2026Review
- Adaptive P-element Insertions in a Long Non-Coding RNA are Potential Emerging piRNA Clusters.Genome biology and evolution · 2026Article
- Meiotic double-strand DNA breaks and spontaneous mutation in Drosophila melanogaster.G3 (Bethesda, Md.) · 2026Article
- PIWI proteins and piRNAs: key regulators of stem cell biology.Frontiers in cell and developmental biology · 2025Review
- Article
- Modeling early germline immunization after horizontal transfer of transposable elements reveals internal piRNA cluster heterogeneity.BMC biology · 2023Article
- Review
- The control of transcriptional memory by stable mitotic bookmarking.Nature communications · 2022Article
- A transposon expression burst accompanies the activation of Y-chromosome fertility genes during Drosophila spermatogenesis.Nature communications · 2021Article
- Transposon Reactivation in the Germline May Be Useful for Both Transposons and Their Host Genomes.Cells · 2020Review
- A dual role of dLsd1 in oogenesis: regulating developmental genes and repressing transposons.Nucleic acids research · 2020Article
- Trapping a somatic endogenous retrovirus into a germline piRNA cluster immunizes the germline against further invasion.Genome biology · 2019Article
- The interplay between the Argonaute proteins Piwi and Aub within Drosophila germarium is critical for oogenesis, piRNA biogenesis and TE silencing.Nucleic acids research · 2018Article
- Efficient Expression of Genes in theGenetics · 2018Article
- Article
- Comparative Proteomic Profiling Reveals Molecular Characteristics Associated with Oogenesis and Oocyte Maturation during Ovarian Development of Bactrocera dorsalis (Hendel).International journal of molecular sciences · 2017Article
- From Embryo to Adult: piRNA-Mediated Silencing throughout Germline Development inG3 (Bethesda, Md.) · 2017Article
- Defining the purity of exosomes required for diagnostic profiling of small RNA suitable for biomarker discovery.RNA biology · 2017Article
- Article
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Authors and funding
10 authors at 4 institutions in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
During Drosophila oogenesis, transposable element (TE) repression involves the Piwi-interacting RNA (piRNA) pathway which ensures genome integrity for the next generation. We developed a transgenic model to study repression of the Idefix retrotransposon in the germline. Using a candidate gene KD-approach, we identified differences in the spatio-temporal requirements of the piRNA pathway components for piRNA-mediated silencing. Some of them (Aub, Vasa, Spn-E) are necessary in very early stages of oogenesis within the germarium and appear to be less important for efficient TE silencing thereafter. Others (Piwi, Ago3, Mael) are required at all stages of oogenesis. Moreover, during early oogenesis, in the dividing cysts within the germarium, Idefix anti-sense transgenes escape host control, and this is associated with very low piwi expression. Silencing of P-element-based transgenes is also strongly weakened in these cysts. This region, termed the 'Piwiless pocket' or Pilp, may ensure that new TE insertions occur and are transmitted to the next generation, thereby contributing to genome dynamics. In contrast, piRNA-mediated silencing is strong in germline stem cells in which TE mobilization is tightly repressed ensuring the continued production of viable germline cysts.
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