ArticleJournal of experimental botany2014
Dual regulation of cytosolic ascorbate peroxidase (APX) by tyrosine nitration and S-nitrosylation.
Article in Journal of experimental botany, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 124 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
124 citing papers in PubMed, 328 citations in OpenAlex.
- Modulation of GSNOR activity for improved NO homeostasis and flood resilience in plants.Plant signaling & behavior · 2026Review
- Hydrogen Sulphide Signalling in Plants: From Persulfidation to Metabolic Regulation and Stress Response.Plant, cell & environment · 2026Review
- Aboveground whole-plant live HRedox biology · 2026Article
- Hydrogen Gas Enhances Salinity Tolerance in Tomato Seedlings by Regulating the S-Nitrosylation of MEK1.Plant biotechnology journal · 2026Article
- Nitric Oxide Enhances Drought Tolerance inPlants (Basel, Switzerland) · 2026Article
- Integration of NO and HPlant communications · 2026Review
- Effects of High Lithium Concentrations on the Growth, Biomass, Mineral Accumulation, Oxidative Stress, Antioxidant and Gene Expression Response, and DNA Methylation in Sunflower Plants.Plants (Basel, Switzerland) · 2026Article
- Peroxynitrite-mediated tyrosine nitration modulates β-1,3-glucanase activity and potato defense againstFrontiers in plant science · 2026Article
- Functional interactions among H2O2, NO, H2S, and melatonin in the physiology, metabolism, and quality of horticultural Solanaceae.Journal of experimental botany · 2025Review
- Integrating Redox Proteomics and Computational Modeling to Decipher Thiol-Based Oxidative Post-Translational Modifications (oxiPTMs) in Plant Stress Physiology.International journal of molecular sciences · 2025Review
- Nitro-fatty acids-mediated nitroalkylation modulates fine-tuning catalase antioxidant function during salinity stress in plants.Protein science : a publication of the Protein Society · 2025Article
- Progress in Plant Nitric Oxide Studies: Implications for Phytopathology and Plant Protection.International journal of molecular sciences · 2025Review
- Nitro-fatty acids modulate germination onset through S-nitrosothiol metabolism.Plant physiology · 2025Article
- Mechanism of sodium nitroprusside regulating ginseng quality.Scientific reports · 2025Article
- Nitric oxide in plants: an insight on redox activity and responses toward abiotic stress signaling.Plant signaling & behavior · 2024Review
- The evolution of S-nitrosylation detection methodology and the role of protein S-nitrosylation in various cancers.Cancer cell international · 2024Review
- An Overview of the Mechanisms through Which Plants Regulate ROS Homeostasis under Cadmium Stress.Antioxidants (Basel, Switzerland) · 2024Review
- The Key Targets of NO-Mediated Post-Translation Modification (PTM) Highlighting the Dynamic Metabolism of ROS and RNS in Peroxisomes.International journal of molecular sciences · 2024Review
- Biochemical and Functional Profiling of Thioredoxin-Dependent Cytosolic GPX-like Proteins inBiomolecules · 2024Article
- Combined Ascorbic Acid and Mild Heat Treatment to Improve the Quality of Fresh-Cut Carrots.Foods (Basel, Switzerland) · 2024Article
64 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
10 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Post-translational modifications (PTMs) mediated by nitric oxide (NO)-derived molecules have become a new area of research, as they can modulate the function of target proteins. Proteomic data have shown that ascorbate peroxidase (APX) is one of the potential targets of PTMs mediated by NO-derived molecules. Using recombinant pea cytosolic APX, the impact of peroxynitrite (ONOO-) and S-nitrosoglutathione (GSNO), which are known to mediate protein nitration and S-nitrosylation processes, respectively, was analysed. While peroxynitrite inhibits APX activity, GSNO enhances its enzymatic activity. Mass spectrometric analysis of the nitrated APX enabled the determination that Tyr5 and Tyr235 were exclusively nitrated to 3-nitrotyrosine by peroxynitrite. Residue Cys32 was identified by the biotin switch method as S-nitrosylated. The location of these residues on the structure of pea APX reveals that Tyr235 is found at the bottom of the pocket where the haem group is enclosed, whereas Cys32 is at the ascorbate binding site. Pea plants grown under saline (150 mM NaCl) stress showed an enhancement of both APX activity and S-nitrosylated APX, as well as an increase of H2O2, NO, and S-nitrosothiol (SNO) content that can justify the induction of the APX activity. The results provide new insight into the molecular mechanism of the regulation of APX which can be both inactivated by irreversible nitration and activated by reversible S-nitrosylation.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.