ArticleScientific reports2013
Cell electrofusion using nanosecond electric pulses.
Article in Scientific reports, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 51 papers.
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Who cites it
51 citing papers in PubMed.
- Endoluminal Catheter Pulsed Field Ablation for the Treatment of Atherosclerotic Vascular Disease.Annals of biomedical engineering · 2026Article
- Hybrid modeling of electroporation and impedance spectroscopy for label free characterization of stem cells.Scientific reports · 2026Article
- Dendritic cell vaccines: Current research progress, challenges, and opportunities.Genes & diseases · 2026Review
- Integrated electrical modeling of circulating tumor cells for enhanced dielectrophoretic trapping and electroporation.Scientific reports · 2026Article
- Biophysical Considerations in Cell Fusion.Advances in experimental medicine and biology · 2026Review
- Microfluidic Electroporation: Recent Advances and Applications.Advances in experimental medicine and biology · 2026Review
- Nanosecond pulsed electric field applications rejuvenate aging endothelial cells by rescuing mitochondrial-to-nuclear retrograde communication.Regenerative therapy · 2025Article
- Characterization of glioma spheroid viability and metastatic potential following monophasic and biphasic pulsed electric fields.Bioelectrochemistry (Amsterdam, Netherlands) · 2025Article
- Excitation and electroporation in genetically engineered excitable S-HEK cells exposed to electric pulses of different durations.Scientific reports · 2025Article
- The design and engineering of synthetic genomes.Nature reviews. Genetics · 2025Review
- Article
- Fusion of Human Synovium-Derived Mesenchymal Stem/Stromal Cells with Primary Human Chondrocytes Using the Modified Adherence Method (MAM).Methods in molecular biology (Clifton, N.J.) · 2025Article
- Feasibility Study for the Use of Gene Electrotransfer and Cell Electrofusion as a Single-Step Technique for the Generation of Activated Cancer Cell Vaccines.The Journal of membrane biology · 2024Article
- Developing Advanced Chimeric Cell Therapy for Duchenne Muscular Dystrophy.International journal of molecular sciences · 2024Article
- Genetically engineered HEK cells as a valuable tool for studying electroporation in excitable cells.Scientific reports · 2024Article
- Article
- Theoretical analysis for the fluctuation in the electric parameters of the electroporated cells before and during the electrofusion.Medical & biological engineering & computing · 2022Article
- Size-Based Sorting and In Situ Clonal Expansion of Single Cells Using Microfluidics.Biosensors · 2022Article
- Gene Electrotransfer into Mammalian Cells Using Commercial Cell Culture Inserts with Porous Substrate.Pharmaceutics · 2022Article
- Substantially Improved Electrofusion Efficiency of Hybridoma Cells: Based on the Combination of Nanosecond and Microsecond Pulses.Bioengineering (Basel, Switzerland) · 2022Article
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Electrofusion is an efficient method for fusing cells using short-duration high-voltage electric pulses. However, electrofusion yields are very low when fusion partner cells differ considerably in their size, since the extent of electroporation (consequently membrane fusogenic state) with conventionally used microsecond pulses depends proportionally on the cell radius. We here propose a new and innovative approach to fuse cells with shorter, nanosecond (ns) pulses. Using numerical calculations we demonstrate that ns pulses can induce selective electroporation of the contact areas between cells (i.e. the target areas), regardless of the cell size. We then confirm experimentally on B16-F1 and CHO cell lines that electrofusion of cells with either equal or different size by using ns pulses is indeed feasible. Based on our results we expect that ns pulses can improve fusion yields in electrofusion of cells with different size, such as myeloma cells and B lymphocytes in hybridoma technology.
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