Evidence map›Paper›PMID 24286326›Full record

ArticleBMB reports2014

Replication and packaging of Turnip yellow mosaic virus RNA containing Flock house virus RNA1 sequence.

Hui-Bae Kim, Do-Yeong Kim, Tae-Ju Cho

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMB reports, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
0.5field-weighted citation impact, top 25% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 3 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 1 institution in 1 country.

Hui-Bae KimDepartment of Biochemistry, Chungbuk National University, Cheongju 361-763, Korea.
Do-Yeong KimDepartment of Biochemistry, Chungbuk National University, Cheongju 361-763, Korea.
Tae-Ju ChoDepartment of Biochemistry, Chungbuk National University, Cheongju 361-763, Korea.
Chungbuk National University · KR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Turnip yellow mosaic virus (TYMV) is a spherical plant virus that has a single 6.3 kb positive strand RNA as a genome. In this study, RNA1 sequence of Flock house virus (FHV) was inserted into the TYMV genome to test whether TYMV can accommodate and express another viral entity. In the resulting construct, designated TY-FHV, the FHV RNA1 sequence was expressed as a TYMV subgenomic RNA. Northern analysis of the Nicotiana benthamiana leaves agroinfiltrated with the TY-FHV showed that both genomic and subgenomic FHV RNAs were abundantly produced. This indicates that the FHV RNA1 sequence was correctly expressed and translated to produce a functional FHV replicase. Although these FHV RNAs were not encapsidated, the FHV RNA having a TYMV CP sequence at the 3'-end was efficiently encapsidated. When an eGFP gene was inserted into the B2 ORF of the FHV sequence, a fusion protein of B2-eGFP was produced as expected.

Indexed as

Agrobacterium tumefaciensCapsid ProteinsGreen Fluorescent ProteinsNicotianaPlant LeavesPlasmidsRecombinant Fusion ProteinsRNA, BacterialRNA-Dependent RNA PolymeraseTymovirusVirus AssemblyVirus ReplicationCapsid Proteinsenhanced green fluorescent proteinGreen Fluorescent ProteinsRecombinant Fusion ProteinsRNA, BacterialRNA-Dependent RNA PolymeraseRNA I

Identifiers

PMID24286326
PMCPMC4163869
OpenAlexW2071646618

What OpenQuestion holds

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LicenceCC BY-NC
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.