ArticleBiotechnology for biofuels2013
Raw starch conversion by Saccharomyces cerevisiae expressing Aspergillus tubingensis amylases.
Article in Biotechnology for biofuels, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 72 citations in OpenAlex.
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- Construction of an amylolyticFrontiers in microbiology · 2024Article
- Evaluation of Shifts of Gene Transcription Levels of Unicellular Green AlgaMicroorganisms · 2023Article
- Genetic Engineering of Starch Biosynthesis in Maize Seeds for Efficient Enzymatic Digestion of Starch during Bioethanol Production.International journal of molecular sciences · 2023Review
- Promoter-proximal introns impact recombinant amylase expression in Saccharomyces cerevisiae.FEMS yeast research · 2023Article
- Characterization and modulation of endoplasmic reticulum stress response target genes in Kluyveromyces marxianus to improve secretory expressions of heterologous proteins.Biotechnology for biofuels · 2021Article
- Bacterial α-diglucoside metabolism: perspectives and potential for biotechnology and biomedicine.Applied microbiology and biotechnology · 2021Review
- Selection of Superior Yeast Strains for the Fermentation of Lignocellulosic Steam-Exploded Residues.Frontiers in microbiology · 2021Article
- NaturalFrontiers in microbiology · 2021Article
- Consolidated Bioprocess for Bioethanol Production from Raw Flour ofMicroorganisms · 2019Article
- Construction of industrialBiotechnology for biofuels · 2019Article
- Isolation, Characterization, and Metal Response of Novel, Acid-Tolerant Penicillium spp. from Extremely Metal-Rich Waters at a Mining Site in Transbaikal (Siberia, Russia).Microbial ecology · 2018Article
- Mating of natural Saccharomyces cerevisiae strains for improved glucose fermentation and lignocellulosic inhibitor tolerance.Folia microbiologica · 2018Article
- Article
- Using a vector pool containing variable-strength promoters to optimize protein production in Yarrowia lipolytica.Microbial cell factories · 2017Article
- Efficient hydrolysis of raw starch and ethanol fermentation: a novel raw starch-digesting glucoamylase fromBiotechnology for biofuels · 2016Article
- Heterologous, Expression, and Characterization of Thermostable Glucoamylase Derived from Aspergillus flavus NSH9 in Pichia pastoris.BioMed research international · 2016Article
- Direct ethanol production from starch using a natural isolate, Scheffersomyces shehatae: Toward consolidated bioprocessing.Scientific reports · 2015Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundStarch is one of the most abundant organic polysaccharides available for the production of bio-ethanol as an alternative transport fuel. Cost-effective utilisation of starch requires consolidated bioprocessing (CBP) where a single microorganism can produce the enzymes required for hydrolysis of starch, and also convert the glucose monomers to ethanol.
resultsThe Aspergillus tubingensis T8.4 α-amylase (amyA) and glucoamylase (glaA) genes were cloned and expressed in the laboratory strain Saccharomyces cerevisiae Y294 and the semi-industrial strain, S. cerevisiae Mnuα1. The recombinant AmyA and GlaA displayed protein sizes of 110-150 kDa and 90 kDa, respectively, suggesting significant glycosylation in S. cerevisiae. The Mnuα1[AmyA-GlaA] and Y294[AmyA-GlaA] strains were able to utilise 20 g l-1 raw corn starch as sole carbohydrate source, with ethanol titers of 9.03 and 6.67 g l-1 (0.038 and 0.028 g l-1 h-1), respectively, after 10 days. With a substrate load of 200 g l-1 raw corn starch, Mnuα1[AmyA-GlaA] yielded 70.07 g l-1 ethanol (0.58 g l-1 h-1) after 120 h of fermentation, whereas Y294[AmyA-GlaA] was less efficient at 43.33 g l-1 ethanol (0.36 g l-1 h-1).
conclusionsIn a semi-industrial amylolytic S. cerevisiae strain expressing the A. tubingensis α-amylase and glucoamylase genes, 200 g l-1 raw starch was completely hydrolysed (saccharified) in 120 hours with 74% converted to released sugars plus fermentation products and the remainder presumably to biomass. The single-step conversion of raw starch represents significant progress towards the realisation of CBP without the need for any heat pretreatment. Furthermore, the amylases were produced and secreted by the host strain, thus circumventing the need for exogenous amylases.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.