ArticleThe EMBO journal1986
Amino acid sequence and gene organization of cytokeratin no. 19, an exceptional tail-less intermediate filament protein.
Article in The EMBO journal, 1986. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 55 papers, 1 of them a synthesis that pooled it.
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Who cites it
55 citing papers in PubMed, 1 synthesis or guideline pooled it, 165 citations in OpenAlex.
- Prognostic significance of cytokeratin 19 expression in pancreatic neuroendocrine tumor: A meta-analysis.PloS one · 2017Pooled it
- Cytokeratin expression in breast cancer: from mechanisms, progression, diagnosis, and prognosis to therapeutic implications.Molecular & cellular oncology · 2025Review
- Postoperative Adjuvant Transcatheterarterial Chemoembolization Should Be Considered Selectively for Patients with Cytokeratin-19 Positive Hepatocellular Carcinoma.Journal of hepatocellular carcinoma · 2025Article
- Review
- Association of CK19 expression with the efficacy of adjuvant transarterial chemoembolization after hepatic resection in hepatocellular carcinoma patients at high risk of recurrence.Journal of clinical and translational research · 2022Article
- CK19 Promotes Ovarian Cancer Development by Impacting on Wnt/β-Catenin Pathway.OncoTargets and therapy · 2020Article
- History, molecular features, and clinical importance of conventional serum biomarkers in lung cancer.Surgery today · 2017Review
- Keratin 19 as a key molecule in progression of human hepatocellular carcinomas through invasion and angiogenesis.BMC cancer · 2016Article
- Cytokeratin19 induced by HER2/ERK binds and stabilizes HER2 on cell membranes.Cell death and differentiation · 2015Article
- Article
- Structure and functions of keratin proteins in simple, stratified, keratinized and cornified epithelia.Journal of anatomy · 2009Review
- The human keratins: biology and pathology.Histochemistry and cell biology · 2008Review
- Congruence of tissue expression profiles from Gene Expression Atlas, SAGEmap and TissueInfo databases.BMC genomics · 2003Article
- Real-time observation of coiled-coil domains and subunit assembly in intermediate filaments.The Journal of biological chemistry · 2002Article
- Cytokeratins 8 and 19 in the mouse placental development.The Journal of cell biology · 2000Article
- Lessons from keratin 18 knockout mice: formation of novel keratin filaments, secondary loss of keratin 7 and accumulation of liver-specific keratin 8-positive aggregates.The Journal of cell biology · 1998Article
- The apical submembrane cytoskeleton participates in the organization of the apical pole in epithelial cells.The Journal of cell biology · 1997Article
- Intermediate filaments in the nervous system: implications in cancer.Cancer metastasis reviews · 1996Review
- Intermediate filament expression in prostate cancer.Cancer metastasis reviews · 1996Review
- Reverse transcriptase/polymerase chain reaction detection of cytokeratin-19 mRNA in bone marrow and blood of breast cancer patients.Journal of cancer research and clinical oncology · 1996Article
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have isolated a cDNA clone from a bovine bladder urothelium library which encodes the smallest intermediate filament (IF) protein known, i.e. the simple epithelial cytokeratin (equivalent to human cytokeratin 19) previously thought to have mol. wt 40,000. This clone was then used to isolate the corresponding gene from which we have determined the complete nucleotide sequence and deduced the amino acid sequence of the encoded protein. This cytokeratin of 399 amino acids (mol. wt 43,893) is identified as a typical acidic (type I) cytokeratin but differs from all other IF proteins in that it does not show the carboxyterminal, non-alpha-helical tail domain. Instead it contains a 13 amino acids extension of the alpha-helical rod. The gene encoding cytokeratin 19 is also exceptional. It contains only five introns which occur in positions corresponding to intron positions in other IF protein genes. However, an intron which in all other IF proteins demarcates the region corresponding to the transition from the alpha-helical rod into the non-alpha-helical tail is missing in the cytokeratin 19 gene. Using in vitro reconstitution of purified cytokeratin 19 we show that it reacts like other type I cytokeratins in that it does not form, in the absence of a type II cytokeratin partner, typical IF. Instead it forms 40-90 nm rods of 10-11 nm diameter which appear to represent lateral associations of a number of cytokeratin molecules. Our results demonstrate that the non-alpha-helical tail domain is not an indispensable feature of IF proteins. The gene structure of this protein provides a remarkable case of a correlation of a change in protein conformation with an exon boundary.
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