ArticleAvicenna journal of medical biotechnology2013
Micropatterning of ECM Proteins on Glass Substrates to Regulate Cell Attachment and Proliferation.
Article in Avicenna journal of medical biotechnology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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Who cites it
4 citing papers in PubMed, 11 citations in OpenAlex.
- Radical-Mediated, Substrate-Independent Fabrication of Hybrid Solid-Hydrogel Materials With Tunable Crosslinking: An Initiator- and Crosslinker-Free Approach.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Plasma-Activated Coated Glass: A Novel Platform for Optimal Optical Performance and Cell Culture Substrate Customization.Small science · 2024Article
- Influence of Micropatterned Grill Lines onFrontiers in cellular and infection microbiology · 2018Article
- Biocompatible and biodegradable fibrinogen microspheres for tumor-targeted doxorubicin delivery.International journal of nanomedicine · 2015Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundMicropatterning is becoming a powerful tool for studying cells in vitro. This method not only uses very small amount of material but also mimic the microenvironment structure present in living tissues better than flask culturing techniques. In previous studies using micropatterning of extracellular matrix proteins on glass surfaces, the rate of protein detachment from the surface was so high that the proteins and the cultivated cells detached after 3 three days of cell seeding.
methodsHere we optimized the glass surface modification method to fulfill the requirement of most in vitro studies.
resultsIn our study we showed that the optimum time for glass surface modification reaction is 1.5 hr, and the cells could be tracked in vitro for over 15 days after cell seeding which is enough for the most in vitro studies. As a model, we cultivated HEK 293T and HepG2 cells on the collagen micro-patterns and showed that they have normal growth and morphology on these micropatterns. The HEK cells also transfected with pmaxGFP plasmid vector to show that the cells on collagen micropatterns could also used in transfection studies.
conclusionTaking these together, this novel method is promising for efficient cell culture studies on micropatterened surfaces in the future.
Indexed as
Identifiers
24285998PMC3838768W2118072247What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.