ArticlePlant physiology2014
An RNA sequencing transcriptome analysis reveals novel insights into molecular aspects of the nitrate impact on the nodule activity of Medicago truncatula.
Article in Plant physiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.
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43 citing papers in PubMed, 99 citations in OpenAlex.
- How Plants May Maintain Protein Homeostasis Under Rising Atmospheric COPlant, cell & environment · 2026Review
- Diverse triggers, common outcome: Senescence in Fix⁻ Medicago truncatula nodules.Plant physiology · 2025Article
- The indirect effect of nitrate on the soybean nodule growth and nitrogen fixation activity in relation to carbon supply.BMC plant biology · 2025Article
- Symbiotic nitrogen fixation: a launchpad for investigating old and new challenges.Journal of experimental botany · 2025Article
- EARLY NODULIN93 acts via cytochrome c oxidase to alter respiratory ATP production and root growth in plants.The Plant cell · 2024Article
- NPF and NRT2 fromPlants (Basel, Switzerland) · 2024Review
- Effects of Elevated Temperature onInternational journal of molecular sciences · 2023Article
- The NAC transcription factors SNAP1/2/3/4 are central regulators mediating high nitrogen responses in mature nodules of soybean.Nature communications · 2023Article
- Improving phosphorus acquisition efficiency through modification of root growth responses to phosphate starvation in legumes.Frontiers in plant science · 2023Review
- Cultivation ofFrontiers in plant science · 2023Article
- Systemic regulation of nodule structure and assimilated carbon distribution by nitrate in soybean.Frontiers in plant science · 2023Article
- Distinct roles of the meningeal layers in CNS autoimmunity.Nature neuroscience · 2022Article
- The lung microbiome regulates brain autoimmunity.Nature · 2022Article
- In Vivo Imaging and Quantification of Carbon Tracer Dynamics in Nodulated Root Systems of Pea Plants.Plants (Basel, Switzerland) · 2022Article
- Systemic regulation of soybean nodulation and nitrogen fixation by nitrogen via isoflavones.Frontiers in plant science · 2022Article
- The Nitrate Transporter MtNPF6.8 Is a Master Sensor of Nitrate Signal in the Primary Root Tip ofFrontiers in plant science · 2022Article
- What signals the glyoxalase pathway in plants?Physiology and molecular biology of plants : an international journal of functional plant biology · 2021Review
- Characteristics and Research Progress of Legume Nodule Senescence.Plants (Basel, Switzerland) · 2021Review
- Article
- TheFrontiers in plant science · 2021Article
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Authors and funding
10 authors at 1 institution in 2 countries.
Funding
Abstract
The mechanism through which nitrate reduces the activity of legume nodules is controversial. The objective of the study was to follow Medicago truncatula nodule activity after nitrate provision continuously and to identify molecular mechanisms, which down-regulate the activity of the nodules. Nodule H2 evolution started to decline after about 4 h of nitrate application. At that point in time, a strong shift in nodule gene expression (RNA sequencing) had occurred (1,120 differentially expressed genes). The most pronounced effect was the down-regulation of 127 genes for nodule-specific cysteine-rich peptides. Various other nodulins were also strongly down-regulated, in particular all the genes for leghemoglobins. In addition, shifts in the expression of genes involved in cellular iron allocation and mitochondrial ATP synthesis were observed. Furthermore, the expression of numerous genes for the formation of proteins and glycoproteins with no obvious function in nodules (e.g. germins, patatin, and thaumatin) was strongly increased. This occurred in conjunction with an up-regulation of genes for proteinase inhibitors, in particular those containing the Kunitz domain. The additionally formed proteins might possibly be involved in reducing nodule oxygen permeability. Between 4 and 28 h of nitrate exposure, a further reduction in nodule activity occurred, and the number of differentially expressed genes almost tripled. In particular, there was a differential expression of genes connected with emerging senescence. It is concluded that nitrate exerts rapid and manifold effects on nitrogenase activity. A certain degree of nitrate tolerance might be achieved when the down-regulatory effect on late nodulins can be alleviated.
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