ArticleClinical and vaccine immunology : CVI2014
Development of a highly sensitive and specific blastomycosis antibody enzyme immunoassay using Blastomyces dermatitidis surface protein BAD-1.
Article in Clinical and vaccine immunology : CVI, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers, 1 of them a synthesis that pooled it.
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Who cites it
38 citing papers in PubMed, 1 synthesis or guideline pooled it, 84 citations in OpenAlex.
- Microbiological Laboratory Testing in the Diagnosis of Fungal Infections in Pulmonary and Critical Care Practice. An Official American Thoracic Society Clinical Practice Guideline.American journal of respiratory and critical care medicine · 2019Guideline
- Performance of Laboratory Testing in Culture-Confirmed Blastomycosis: A Retrospective Study.Open forum infectious diseases · 2026Article
- Blastomyces Urine Antigen Testing for Active Case Identification During a Blastomycosis Outbreak.Emerging infectious diseases · 2026Article
- Role of Blastomyces BAD-1 IgG Enzyme Immunoassay (EIA) for the diagnosis of blastomycosis in persons residing in an endemic area.Medical mycology · 2026Article
- Diagnosing Blastomycosis: A Review of Laboratory Methods and Clinical Utility.Journal of fungi (Basel, Switzerland) · 2025Review
- Agglutination of yeast-binding antibodies from human blood plasma products.BMC microbiology · 2025Article
- Practical Guidance for Clinical Microbiology Laboratories: Antibody and antigen detection methods for dimorphic fungal infections.Clinical microbiology reviews · 2025Review
- Perspectives on blastomycosis in Canada in the face of climate change.Canada communicable disease report = Releve des maladies transmissibles au Canada · 2024Article
- Blastomycosis: A Review of Mycological and Clinical Aspects.Journal of fungi (Basel, Switzerland) · 2023Review
- Genitourinary Blastomycosis in a Young Male Patient: A Case Report and Review of Diagnostic Challenges.Case reports in infectious diseases · 2023Article
- Immunologic Diagnosis of Endemic Mycoses.Journal of fungi (Basel, Switzerland) · 2022Review
- Blastomycosis-Some Progress but Still Much to Learn.Journal of fungi (Basel, Switzerland) · 2022Review
- Blastomycosis Presenting With Acute Airway Obstruction From a Retropharyngeal Abscess and Complicated by Severe Hypokalemia During Posaconazole Therapy: A Case Report and Review of Literature.Open forum infectious diseases · 2022Article
- Diagnostic Methods and Risk Factors for Severe Disease and Mortality in Blastomycosis: A Retrospective Cohort Study.Journal of fungi (Basel, Switzerland) · 2021Article
- Diagnosis of Pulmonary Infections Due to Endemic Fungi.Diagnostics (Basel, Switzerland) · 2021Review
- Current and New Perspectives in the Diagnosis of Blastomycosis and Histoplasmosis.Journal of fungi (Basel, Switzerland) · 2020Review
- Advances in the diagnosis of fungal pneumonias.Expert review of respiratory medicine · 2020Review
- Persistent and nonprogressive cutaneous blastomycosis in a pregnant adolescent.JAAD case reports · 2020Article
- Close encounters of three kinds: Case-based report of endemic mycoses in the environs of New York city.Respiratory medicine case reports · 2020Article
- [Pulmonary blastomycosis].The Pan African medical journal · 2020Article
Corrections and comments
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Authors and funding
10 authors at 3 institutions in 1 country.
Funding
Abstract
Serologic tests for antibodies to Blastomyces dermatitidis are not thought to be useful for the diagnosis of blastomycosis, in part due to the low sensitivity of immunodiffusion and complement fixation. Earlier studies have shown that the enzyme immunoassay improves the sensitivity of antibody detection for the diagnosis of blastomycosis. Microplates coated with the B. dermatitidis surface protein BAD-1 were used for testing sera from patients with proven blastomycosis or histoplasmosis and controls. Semiquantification was accomplished by using standards containing human anti-B. dermatitidis antibodies. The antibodies were detected in 87.8% of the patients with blastomycosis by the enzyme immunoassay compared to 15.0% by immunodiffusion. The specificities were 99.2% for patients with nonfungal infections and healthy subjects and 94.0% for patients with histoplasmosis. The results were highly reproducible on repeat testing. When combined with antigen testing, antibody testing improved the sensitivity from 87.8% to 97.6%. Enzyme immunoassay detection of antibodies against BAD-1 is highly specific, has greatly improved sensitivity over immunodiffusion, and may identify cases with negative results by antigen testing. This assay has the potential to aid in the diagnosis of blastomycosis.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.