ArticleBiology of reproduction2014
Inhibitory effect of progesterone on cervical tissue formation in a three-dimensional culture system with human cervical fibroblasts.
Article in Biology of reproduction, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
16 citing papers in PubMed, 28 citations in OpenAlex.
- The Silent Cost of Gender in Mitral Valve Surgery: A Propensity-Score Matched Analysis.European journal of cardio-thoracic surgery : official journal of the European Association for Cardio-thoracic Surgery · 2026Article
- Risk factors for spontaneous preterm birth are mediated through changes in cervical length.Communications medicine · 2025Article
- Ovarian steroids modulate mRNA expression of ECM associated genes and collagen deposition induced by TGF β1 in equine endometrium in vitro.Scientific reports · 2025Article
- Stem-Cell-Regenerative and Protective Effects of Squid (Marine drugs · 2024Article
- Bioengineering and the cervix: The past, current, and future for addressing preterm birth.Current research in physiology · 2023Review
- Progesterone and its receptor signaling in cervical remodeling: Mechanisms of physiological actions and therapeutic implications.The Journal of steroid biochemistry and molecular biology · 2022Review
- Collagen and elastic fiber remodeling in the pregnant mouse myometrium†.Biology of reproduction · 2022Article
- Tissue Engineering for Cervical Function in Pregnancy.Current opinion in biomedical engineering · 2022Article
- Review
- Bioengineering of the Uterus.Reproductive sciences (Thousand Oaks, Calif.) · 2021Review
- Mechanical and Biochemical Effects of Progesterone on Engineered Cervical Tissue.Tissue engineering. Part A · 2018Article
- Cervical alterations in pregnancy.Best practice & research. Clinical obstetrics & gynaecology · 2018Review
- Steroid Hormones Are Key Modulators of Tissue Mechanical Function via Regulation of Collagen and Elastic Fibers.Endocrinology · 2017Article
- Agonist-Dependent Downregulation of Progesterone Receptors in Human Cervical Stromal Fibroblasts.Reproductive sciences (Thousand Oaks, Calif.) · 2016Article
- Vaginal progesterone, but not 17α-hydroxyprogesterone caproate, has antiinflammatory effects at the murine maternal-fetal interface.American journal of obstetrics and gynecology · 2015Article
- Progestin-Containing Contraceptives Alter Expression of Host Defense-Related Genes of the Endometrium and Cervix.Reproductive sciences (Thousand Oaks, Calif.) · 2015Article
Corrections and comments
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Authors and funding
5 authors at 4 institutions in 1 country.
Funding
Abstract
Progesterone supplementation is recommended to prevent preterm birth in women with a short cervix, but the mechanism is unclear. We hypothesize that progesterone acts by altering the composition of the cervical extracellular matrix (ECM). We tested this hypothesis using human cervical fibroblasts in both two-dimensional (2D) and three-dimensional (3D) cultures. For 2D culture, cells were seeded in 6-well plates and cultured with media supplemented with estradiol (10(-8) M), progesterone (10(-7) or 10(-6) M), and vehicle. For 3D culture, the cells were cultured on a porous silk protein scaffold system. Progesterone and estrogen receptors were documented by immunohistochemistry and Western blot analysis. In both 2D and 3D cultures, decreased collagen synthesis was seen with increased progesterone concentration. Three-dimensional cultures could be maintained significantly longer than 2D cultures, and the morphology of 3D cultures appeared similar to native cervical tissue. Thus, further studies were performed in 3D culture. To determine the effect of progesterone concentration, the 3D scaffolds were cultured with estradiol (10(-8) M) and five conditions: vehicle; 10(-9), 10(-8), or 10(-7) M progesterone; or 10(-7) M progesterone plus 10(-6) M mifepristone. The highest progesterone concentration correlated with the least amount of collagen synthesis. Collagen synthesis progressively increased as progesterone concentration decreased. This effect was partially antagonized by mifepristone, suggesting the mechanism is mediated by the progesterone receptor. This hormonally responsive 3D culture system supports the hypothesis that progesterone has a direct effect on remodeling cervical ECM during pregnancy. The 3D culture system could be useful for studying the mechanism of progesterone effects on the cervix.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.