ArticleCytoskeleton (Hoboken, N.J.)2014
Eg5 restricts anaphase B spindle elongation in mammalian cells.
Article in Cytoskeleton (Hoboken, N.J.), 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
24 citing papers in PubMed, 38 citations in OpenAlex.
- Midzone bundles of the mammalian anaphase spindle are mechanically coupled both locally and globally.bioRxiv : the preprint server for biology · 2026Article
- Lateral and longitudinal compaction of PRC1 overlap zones drives stabilization of interzonal microtubules.Molecular biology of the cell · 2023Article
- Modest increase of KIF11 expression exposes fragilities in the mitotic spindle, causing chromosomal instability.Journal of cell science · 2022Article
- Kinesin-5 Eg5 mediates centrosome separation to control spindle assembly in spermatocytes.Chromosoma · 2022Article
- Kinesin-14 HSET may not oppose kinesin-5 Eg5 activity in RPE-1 cells.microPublication biology · 2022Article
- An anaphase surveillance mechanism prevents micronuclei formation from frequent chromosome segregation errors.Cell reports · 2021Article
- Anaphase B: Long-standing models meet new concepts.Seminars in cell & developmental biology · 2021Review
- Mechanisms by Which Kinesin-5 Motors Perform Their Multiple Intracellular Functions.International journal of molecular sciences · 2021Review
- Microtubule-sliding modules based on kinesins EG5 and PRC1-dependent KIF4A drive human spindle elongation.Developmental cell · 2021Article
- Small molecule allosteric uncoupling of microtubule depolymerase activity from motility in human Kinesin-5 during mitotic spindle assembly.Scientific reports · 2019Article
- High-resolution imaging reveals how the spindle midzone impacts chromosome movement.The Journal of cell biology · 2019Article
- Mechanisms of Spindle Positioning: Lessons from Worms and Mammalian Cells.Biomolecules · 2019Review
- NEK7 regulates dendrite morphogenesis in neurons via Eg5-dependent microtubule stabilization.Nature communications · 2018Article
- Bidirectional motility of kinesin-5 motor proteins: structural determinants, cumulative functions and physiological roles.Cellular and molecular life sciences : CMLS · 2018Review
- Eg5 orchestrates porcine oocyte maturational progression by maintaining meiotic organelle arrangement.Cell division · 2018Article
- Microtubule Sliding within the Bridging Fiber Pushes Kinetochore Fibers Apart to Segregate Chromosomes.Developmental cell · 2017Article
- Eg5 Inhibitors Have Contrasting Effects on Microtubule Stability and Metaphase Spindle Integrity.ACS chemical biology · 2017Article
- Anaphase B.Biology · 2016Review
- Kinesin-5 inhibitor resistance is driven by kinesin-12.The Journal of cell biology · 2016Article
- Direct interaction between centralspindlin and PRC1 reinforces mechanical resilience of the central spindle.Nature communications · 2015Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
During anaphase, overlapping antiparallel microtubules in the spindle interzone elongate and contribute to chromosome segregation. Kinesin-5 family members are required for spindle elongation in some cells, but in other cases they restrict elongation acting like a brake. To determine how kinesin-5 contributes to spindle elongation in mammalian cells, we treated LLC-Pk1 epithelial cells with small molecule inhibitors of the mammalian kinesin-5, Eg5, at anaphase onset and measured the rate and extent of spindle pole separation using multidimensional tracking of centrosomes in cells expressing GFP-γ-tubulin. Centrosome separation was biphasic, with an initial fast phase followed by a slower phase. Treatment with the small molecule inhibitor, STLC, which weakens the interaction of Eg5 with microtubules, resulted in an increase in the rate of centrosome separation. Conversely, treatment with FCPT, which induces a rigor-like interaction of Eg5 with microtubules, reduced the rate of spindle elongation. In control cells, GFP-Eg5 was localized to spindle microtubules and accumulated in the interzone as anaphase progressed. Spindle fluorescence of GFP-Eg5 was decreased following treatment with STLC and increased in cells treated with FCPT. In anaphase cells, cortical dynein increases and rocking motion of spindle poles was detected consistent with the possibility that dynein mediates spindle elongation. In summary, our results demonstrate that Eg5 is not required for spindle elongation, and in fact, restricts the rate of spindle elongation in mammalian cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.