ArticleAmerican journal of physiology. Renal physiology2014
Purinergic and muscarinic modulation of ATP release from the urothelium and its paracrine actions.
Article in American journal of physiology. Renal physiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers.
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Who cites it
44 citing papers in PubMed, 79 citations in OpenAlex.
- Cellular signaling pathway of Shiga toxin-induced ATP release.Frontiers in cellular and infection microbiology · 2026Article
- Contractions of ex-vivo mouse bladder driven by activation of channelrhodopsin-2 expressed in urothelial cells.Scientific reports · 2025Article
- Prostaglandins Regulate Urinary Purines by Modulating Soluble Nucleotidase Release in the Bladder Lumen.International journal of molecular sciences · 2025Article
- Mechanosensitive release of ATP in the urinary bladder mucosa.Purinergic signalling · 2025Review
- Electroacupuncture improves cyclophosphamide-induced bladder overactivity by reducing mechanotransduction in the rat urothelium.Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan · 2025Article
- Connexins 43 and 45 hemichannels mediate ATP release in the urinary bladder.Bladder (San Francisco, Calif.) · 2025Article
- Cholinergic sensing of allergen exposure by airway epithelium promotes type 2 immunity in the lungs.The Journal of allergy and clinical immunology · 2024Article
- Review
- The role of intracellular calcium and Rho kinase pathways in G protein-coupled receptor-mediated contractions of urinary bladder urothelium and lamina propria.American journal of physiology. Cell physiology · 2023Article
- Urinary ATP Levels Are Controlled by Nucleotidases Released from the Urothelium in a Regulated Manner.Metabolites · 2022Article
- Transient receptor potential vanilloid type 4 (TRPV4) in urinary bladder structure and function.Current topics in membranes · 2022Review
- Differential Regulation of Allergic Airway Inflammation by Acetylcholine.Frontiers in immunology · 2022Article
- Purinergic signalling in the urinary bladder - When function becomes dysfunction.Autonomic neuroscience : basic & clinical · 2021Article
- The Urothelium: Life in a Liquid Environment.Physiological reviews · 2020Review
- LPS-mediated release of ATP from urothelial cells occurs by lysosomal exocytosis.Neurourology and urodynamics · 2020Article
- Investigating the associations of mucosal P2Y6 receptor expression and urinary ATP and ADP concentrations, with symptoms of overactive bladder.Neurourology and urodynamics · 2020Article
- TRPV4 receptor as a functional sensory molecule in bladder urothelium: Stretch-independent, tissue-specific actions and pathological implications.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2020Article
- TRPV4 blockade reduces voiding frequency, ATP release, and pelvic sensitivity in mice with chronic urothelial overexpression of NGF.American journal of physiology. Renal physiology · 2019Article
- Purinergic receptor mediated calcium signalling in urothelial cells.Scientific reports · 2019Article
- Intermittent restraint stress induces circadian misalignment in the mouse bladder, leading to nocturia.Scientific reports · 2019Article
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
Abstract
The urothelium is a newly recognized sensory structure that detects bladder fullness. Pivotal to this sensory role is the release of ATP from the urothelium. However, the routes for urothelial ATP release, its modulation by receptor-mediated pathways, and the autocrine/paracrine role of ATP are poorly understood, especially in native tissue. We examined the action of key neurotransmitters: purinergic and muscarinic agonists on ATP release and its paracrine effect. Guinea pig and human urothelial mucosa were mounted in a perfusion trough; superfusate ATP was measured using a luciferin-luciferase assay, and tissue contractions were recorded with a tension transducer. Intracellular Ca²⁺ was measured in isolated urothelial cells with fura-2. The P2Y agonist UTP but not the P2X agonist α,β-methylene-ATP generated ATP release. The muscarinic agonist carbachol and the M₂-preferential agonist oxotremorine also generated ATP release, which was antagonized by the M₂-specific agent methoctramine. Agonist-evoked ATP release was accompanied by mucosal contractions. Urothelial ATP release was differentially mediated by intracellular Ca²⁺ release, cAMP, exocytosis, or connexins. Urothelium-attached smooth muscle exhibited spontaneous contractions that were augmented by subthreshold concentrations of carbachol, which had little direct effect on smooth muscle. This activity was attenuated by desensitizing P2X receptors on smooth muscle. Urothelial ATP release was increased in aging bladders. Purinergic and muscarinic agents produced similar effects in human urothelial tissue. This is the first demonstration of specific modulation of urothelial ATP release in native tissue by purinergic and muscarinic neurotransmitters via distinct mechanisms. Released ATP produces paracrine effects on underlying tissues. This process is altered during aging and has relevance to human bladder pathologies.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.