ArticleNucleic acids research2014
Syn5 RNA polymerase synthesizes precise run-off RNA products.
Article in Nucleic acids research, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 27 citations in OpenAlex.
- IntegratedMolecular therapy. Nucleic acids · 2026Article
- Efficient production of a self-amplifying RNA vaccine sequence with the single subunit RNA polymerase fromRNA (New York, N.Y.) · 2026Article
- Fluorescence-Activated Cell Sorting Enables Rapid Screening of RNA Polymerase-Promoter Interaction in E. coli.Biochemistry · 2026Article
- Simultaneous in vitro expression of minimal 21 transfer RNAs by tRNA array method.Nature communications · 2025Article
- A new approach to RNA synthesis: immobilization of stably and functionally co-tethered promoter DNA and T7 RNA polymerase.Nucleic acids research · 2024Article
- Molecular Biology Applications of Psychrophilic Enzymes: Adaptations, Advantages, Expression, and Prospective.Applied biochemistry and biotechnology · 2024Review
- DNA-terminus-dependent transcription by T7 RNA polymerase and its C-helix mutants.Nucleic acids research · 2024Article
- Bacteriophage RNA polymerases: catalysts for mRNA vaccines and therapeutics.Frontiers in molecular biosciences · 2024Review
- Understanding the impact ofFrontiers in molecular biosciences · 2024Review
- Total RNA Synthesis and its Covalent Labeling Innovation.Topics in current chemistry (Cham) · 2022Review
- Psychrophilic phage VSW-3 RNA polymerase reduces both terminal and full-length dsRNA byproducts inRNA biology · 2022Article
- Regulatory Considerations on the Development of mRNA Vaccines.Current topics in microbiology and immunology · 2022Article
- A single mutation attenuates both the transcription termination and RNA-dependent RNA polymerase activity of T7 RNA polymerase.RNA biology · 2021Article
- Promoter RNA sequencing (PRSeq) for the massive and quantitative promoter analysis in vitro.Scientific reports · 2019Article
- A practical approach to generate suitable de novo synthesis RNA template for a flavivirus RNA-dependent RNA polymerase.Virologica Sinica · 2017Article
- Analysis of RNA structure using small-angle X-ray scattering.Methods (San Diego, Calif.) · 2017Article
- Synthesis of 2'-Fluoro RNA by Syn5 RNA polymerase.Nucleic acids research · 2015Article
- The thumb subdomain of yeast mitochondrial RNA polymerase is involved in processivity, transcript fidelity and mitochondrial transcription factor binding.RNA biology · 2015Article
- Progress and outlook in structural biology of large viral RNAs.Virus research · 2014Review
- Bacteriophage T7 DNA polymerase - sequenase.Frontiers in microbiology · 2014Review
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The enzyme predominantly used for in vitro run-off RNA synthesis is bacteriophage T7 RNA polymerase. T7 RNA polymerase synthesizes, in addition to run-off products of precise length, transcripts with an additional non-base-paired nucleotide at the 3'-terminus (N+1 product). This contaminating product is extremely difficult to remove. We recently characterized the single-subunit RNA polymerase from marine cyanophage Syn5 and identified its promoter sequence. This marine enzyme catalyses RNA synthesis over a wider range of temperature and salinity than does T7 RNA polymerase. Its processivity is >30,000 nt without significant intermediate products. The requirement for the initiating nucleotide at the promoter is less stringent for Syn5 RNA polymerase as compared to T7 RNA polymerase. A major difference is the precise run-off transcripts with homogeneous 3'-termini synthesized by Syn5 RNA polymerase. Therefore, the enzyme is advantageous for the production of RNAs that require precise 3'-termini, such as tRNAs and RNA fragments that are used for subsequent assembly.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.