ArticleAnnals of botany2014
Continuous, high-resolution biospeckle imaging reveals a discrete zone of activity at the root apex that responds to contact with obstacles.
Article in Annals of botany, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed.
- Root system architecture and drought adaptation: emerging tools and genetic insights.Frontiers in plant science · 2026Review
- Early root phenotyping in sweetpotato (PeerJ · 2023Article
- Label-free optical interferometric microscopy to characterize morphodynamics in living plants.Frontiers in plant science · 2023Review
- Monitoring the apical growth characteristics of hairy roots using non-invasive laser speckle contrast imaging.Engineering in life sciences · 2022Article
- In situ laser manipulation of root tissues in transparent soil.Plant and soil · 2021Article
- Biospeckle-characterization of hairy root cultures using laser speckle photometry.Engineering in life sciences · 2020Article
- New live screening of plant-nematode interactions in the rhizosphere.Scientific reports · 2018Article
- RhizoTubes as a new tool for high throughput imaging of plant root development and architecture: test, comparison with pot grown plants and validation.Plant methods · 2016Article
- Length and activity of the root apical meristem revealed in vivo by infrared imaging.Journal of experimental botany · 2015Article
- A scanner system for high-resolution quantification of variation in root growth dynamics of Brassica rapa genotypes.Journal of experimental botany · 2014Article
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6 authors.
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Abstract
BACKGROUND AND
aimsShining a laser onto biological material produces light speckles termed biospeckles. Patterns of biospeckle activity reflect changes in cell biochemistry, developmental processes and responses to the environment. The aim of this work was to develop methods to investigate the biospeckle activity in roots and to characterize the distribution of its intensity and response to thigmostimuli.
methodsBiospeckle activity in roots of Zea mays, and also Jatropha curcas and Citrus limonia, was imaged live and in situ using a portable laser and a digital microscope with a spatial resolution of 10 μm per pixel and the ability to capture images every 0.080 s. A procedure incorporating a Fujii algorithm, image restoration using median and Gaussian filters, image segmentation using maximum-entropy threshold methods and the extraction of features using a tracing algorithm followed by spline fitting were developed to obtain quantitative information from images of biospeckle activity. A wavelet transform algorithm was used for spectral decomposition of biospeckle activity and generalized additive models were used to attribute statistical significance to changes in patterns of biospeckle activity. KEY
resultsThe intensity of biospeckle activity was greatest close to the root apex. Higher frequencies (3-6 Hz) contributed most to the total intensity of biospeckle activity. When a root encountered an obstacle, the intensity of biospeckle activity decreased abruptly throughout the root system. The response became attenuated with repeated thigmostimuli.
conclusionsThe data suggest that at least one component of root biospeckle activity resulted from a biological process, which is located in the zone of cell division and responds to thigmostimuli. However, neither individual cell division events nor root elongation is likely to be responsible for the patterns of biospeckle activity.
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