ArticleScientific reports2013
Hematopoietic stem and progenitor cells acquire distinct DNA-hypermethylation during in vitro culture.
Article in Scientific reports, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 36 citations in OpenAlex.
- Biomimetic Fibrinogen Nanofiber Scaffolds for Vascular Hematopoietic Stem Cell Niche Engineering.Advanced healthcare materials · 2026Article
- Gene regulatory network inference with popInfer reveals the dynamic regulation of hematopoietic stem cell quiescence.iScience · 2025Article
- Variations in Innate Immune Cell Subtypes Correlate with Epigenetic Clocks, Inflammaging and Health Outcomes.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Culture expansion of CAR T cells results in aberrant DNA methylation that is associated with adverse clinical outcome.Leukemia · 2023Article
- Hematopoietic differentiation persists in human iPSCs defective in de novo DNA methylation.BMC biology · 2022Article
- DNA methylation dynamic of bone marrow hematopoietic stem cells after allogeneic transplantation.Stem cell research & therapy · 2019Article
- Therapeutic potential of menstrual blood-derived endometrial stem cells in cardiac diseases.Cellular and molecular life sciences : CMLS · 2019Review
- Tracking of epigenetic changes during hematopoietic differentiation of induced pluripotent stem cells.Clinical epigenetics · 2019Article
- Variants ofLife science alliance · 2018Article
- Tracing human stem cell lineage during development using DNA methylation.Genome research · 2018Article
- Epigenetic aging of human hematopoietic cells is not accelerated upon transplantation into mice.Clinical epigenetics · 2018Article
- The niche for hematopoietic stem cell expansion: a collaboration network.Cellular & molecular immunology · 2017Article
- Elevated expression of DNMT1 is associated with increased expansion and proliferation of hematopoietic stem cells co-cultured with human MSCs.Blood research · 2017Article
- Contribution of epigenetic mechanisms to variation in cancer risk among tissues.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Article
- Systemic epigenetic response to recombinant lentiviral vectors independent of proviral integration.Epigenetics & chromatin · 2016Article
- Hematopoietic stem cell expansion and generation: the ways to make a breakthrough.Blood research · 2015Review
- Article
- PRC2 inhibition counteracts the culture-associated loss of engraftment potential of human cord blood-derived hematopoietic stem and progenitor cells.Scientific reports · 2015Article
- Challenges in identifying the best source of stem cells for cardiac regeneration therapy.Stem cell research & therapy · 2015Review
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Hematopoietic stem and progenitor cells (HPCs) can be maintained in vitro, but the vast majority of their progeny loses stemness during culture. In this study, we compared DNA-methylation (DNAm) profiles of freshly isolated and culture-expanded HPCs. Culture conditions of CD34(+) cells - either with or without mesenchymal stromal cells (MSCs) - had relatively little impact on DNAm, although proliferation is greatly increased by stromal support. However, all cultured HPCs - even those which remained CD34(+) - acquired significant DNA-hypermethylation. DNA-hypermethylation occurred particularly in up-stream promoter regions, shore-regions of CpG islands, binding sites for PU.1, HOXA5 and RUNX1, and it was reflected in differential gene expression and variant transcripts of DNMT3A. Low concentrations of DNAm inhibitors slightly increased the frequency of colony-forming unit initiating cells. Our results demonstrate that HPCs acquire DNA-hypermethylation at specific sites in the genome which is relevant for the rapid loss of stemness during in vitro manipulation.
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