Evidence map›Paper›PMID 24284362›Full record

Trial reportJournal of human genetics2014

The tumor-suppressive microRNA-143/145 cluster inhibits cell migration and invasion by targeting GOLM1 in prostate cancer.

Satoko Kojima, Hideki Enokida, Hirofumi Yoshino, Toshihiko Itesako, Takeshi Chiyomaru, Takashi Kinoshita, Miki Fuse, Rika Nishikawa, Yusuke Goto, Yukio Naya and 2 more

Open access · bronzeAbstract readClinical Trial
PubMed Publisher
In one paragraph

Trial report in Journal of human genetics, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 74 papers.

0numbers the graph read from it
0cells of the map it votes in
74citing papers in PubMed
5.8field-weighted citation impact, top 3% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

74 citing papers in PubMed, 120 citations in OpenAlex.

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14 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 3 institutions in 1 country.

Satoko KojimaDepartment of Urology, Teikyo University Chiba Medical Center, Chiba, Japan.
Hideki EnokidaDepartment of Urology, Graduate School of Medical and Dental Sciences, Kagoshima University, Kagoshima, Japan.
Hirofumi YoshinoDepartment of Urology, Graduate School of Medical and Dental Sciences, Kagoshima University, Kagoshima, Japan.
Toshihiko ItesakoDepartment of Urology, Graduate School of Medical and Dental Sciences, Kagoshima University, Kagoshima, Japan.
Takeshi ChiyomaruDepartment of Urology, Graduate School of Medical and Dental Sciences, Kagoshima University, Kagoshima, Japan.
Takashi KinoshitaDepartment of Functional Genomics, Graduate School of Medicine, Chiba University, Chiba, Japan.
Miki FuseDepartment of Functional Genomics, Graduate School of Medicine, Chiba University, Chiba, Japan.
Rika NishikawaDepartment of Functional Genomics, Graduate School of Medicine, Chiba University, Chiba, Japan.
Yusuke GotoDepartment of Functional Genomics, Graduate School of Medicine, Chiba University, Chiba, Japan.
Yukio NayaDepartment of Urology, Teikyo University Chiba Medical Center, Chiba, Japan.
Masayuki NakagawaDepartment of Urology, Graduate School of Medical and Dental Sciences, Kagoshima University, Kagoshima, Japan.
Naohiko SekiDepartment of Functional Genomics, Graduate School of Medicine, Chiba University, Chiba, Japan.
Chiba University · JPKagoshima University · JPTeikyo University Chiba Medical Center · JP

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Our recent study of microRNA (miRNA) expression signature of prostate cancer (PCa) has revealed that the microRNA-143/145 (miR-143/145) cluster is significantly downregulated in cancer tissues, suggesting that these cluster miRNAs are candidate tumor suppressors. The aim of this study was to investigate the functional significance of the miR-143/145 cluster in PCa cells and to identify novel targets regulated by these cluster miRNAs in PCa. Restoration of miR-143 or miR-145 in PCa cell lines (PC3 and DU145) revealed that these miRNAs significantly inhibited cancer cell migration and invasion. Gene expression data and in silico analysis demonstrated that Golgi membrane protein 1 (GOLM1) resembling a type II golgi transmembrane protein was a potential target of miR-143/145 cluster target gene. Gene expression studies and luciferase reporter assays showed that GOLM1 was directly regulated by the miR-143/145 cluster. Silencing of GOLM1 resulted in significant inhibition of cell migration and invasion in PCa cells. Furthermore, the expression of GOLM1 was upregulated in cancer tissues by immunohistochemistry. Loss of the tumor-suppressive miR-143/145 cluster enhanced cancer cell migration and invasion in PCa through directly regulating GOLM1. Our data on target genes regulated by the tumor-suppressive miR-143/145 cluster provide new insights into the potential mechanisms of PCa oncogenesis and metastasis.

Indexed as

Cell MovementGene Expression RegulationMultigene FamilyAgedCell Line, TumorHumansMaleMembrane ProteinsMicroRNAsMiddle AgedNeoplasm InvasivenessNeoplasm ProteinsProstatic NeoplasmsRNA, NeoplasmGOLM1 protein, humanMembrane ProteinsMicroRNAsMIRN143 microRNA, humanMIRN145 microRNA, humanNeoplasm ProteinsRNA, Neoplasm

Identifiers

PMID24284362
OpenAlexW1985050690

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.