ArticlePeerJ2013
ezRAD: a simplified method for genomic genotyping in non-model organisms.
Article in PeerJ, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 86 papers.
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Who cites it
86 citing papers in PubMed.
- Breeding Behaviors and Kinship in the Endangered Hawaiian Stilt (Ae'o;Ecology and evolution · 2026Article
- Genetic Composition Is Unrelated to Song Content in a Wild Passerine.Molecular ecology · 2026Article
- The complete mitogenome, phylogenetic placement and cox1 variation of Cape sea urchins (Parechinus angulosus) in southern Africa.Molecular biology reports · 2026Article
- Next-Generation Genotyping: Innovations Driving Plant Genomic Improvement.Life (Basel, Switzerland) · 2026Review
- Low genetic differentiation among morphologically distinct Cycas species informs the delineation of conservation management units.Annals of botany · 2026Article
- Inverse Restriction Site-Associated DNA Sequencing (iRAD-seq).Bio-protocol · 2026Article
- A novel method for effectively selecting fragments not associated with restriction sites for whole-genome genotyping.BMC biology · 2025Article
- Article
- Management and conservation implications of cryptic population substructure for two commercially exploited fishes (Merluccius spp.) in southern Africa.Molecular ecology resources · 2025Article
- Optimizing ddRAD sequencing for population genomic studies with ddgRADer.Molecular ecology resources · 2025Article
- Global stock structure of the Silky shark (PeerJ · 2025Article
- Genome-wide SNP Analyses Reveal High Gene Flow of Endemic Smallscale Croaker (Zoological studies · 2025Article
- Article
- The estimation of additive genetic variance of body size in a wild passerine is sensitive to the method used to estimate relatedness among the individuals.Ecology and evolution · 2024Article
- The complete mitochondrial genome of a species ofMitochondrial DNA. Part B, Resources · 2024Article
- Urbanization drives adaptive evolution in a Neotropical bird.Current zoology · 2023Article
- Geographic destiny trumps taxonomy in the Roundtail Chub, Gila robusta species complex (Teleostei, Leuciscidae).Scientific reports · 2023Article
- Genomic evidence indicates small island-resident populations and sex-biased behaviors of Hawaiian reef Manta Rays.BMC ecology and evolution · 2023Article
- The complete mitochondrial genome of the Reef Manta Ray,Mitochondrial DNA. Part B, Resources · 2023Article
- Range expansion and first observation ofEcology and evolution · 2022Article
26 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Here, we introduce ezRAD, a novel strategy for restriction site-associated DNA (RAD) that requires little technical expertise or investment in laboratory equipment, and demonstrate its utility for ten non-model organisms across a wide taxonomic range. ezRAD differs from other RAD methods primarily through its use of standard Illumina TruSeq library preparation kits, which makes it possible for any laboratory to send out to a commercial genomic core facility for library preparation and next-generation sequencing with virtually no additional investment beyond the cost of the service itself. This simplification opens RADseq to any lab with the ability to extract DNA and perform a restriction digest. ezRAD also differs from others in its flexibility to use any restriction enzyme (or combination of enzymes) that cuts frequently enough to generate fragments of the desired size range, without requiring the purchase of separate adapters for each enzyme or a sonication step, which can further decrease the cost involved in choosing optimal enzymes for particular species and research questions. We apply this method across a wide taxonomic diversity of non-model organisms to demonstrate the utility and flexibility of our approach. The simplicity of ezRAD makes it particularly useful for the discovery of single nucleotide polymorphisms and targeted amplicon sequencing in natural populations of non-model organisms that have been historically understudied because of lack of genomic information.
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