ArticlePloS one2013
Establishment of a markerless mutation delivery system in Bacillus subtilis stimulated by a double-strand break in the chromosome.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
21 citing papers in PubMed, 47 citations in OpenAlex.
- Influence of PepF peptidase and sporulation on microcin J25 production inMicrobiology spectrum · 2024Article
- Improved biosynthesis of heme in Bacillus subtilis through metabolic engineering assisted fed-batch fermentation.Microbial cell factories · 2023Article
- Barriers to simultaneous multilocus integration in Bacillus subtilis tumble down: development of a straightforward screening method for the colorimetric detection of one-step multiple gene insertion using the CRISPR-Cas9 system.Microbial cell factories · 2023Article
- Synthetic biology tools for engineeringComputational and structural biotechnology journal · 2023Review
- Improvement of the catalytic performance of glycerol kinase from Bacillus subtilis by chromosomal site-directed mutagenesis.Biotechnology letters · 2022Article
- A facile and robust T7-promoter-based high-expression of heterologous proteins in Bacillus subtilis.Bioresources and bioprocessing · 2022Article
- Plasmid Curing and Exchange Using a Novel Counter-Selectable Marker Based on Unnatural Amino Acid Incorporation at a Sense Codon.International journal of molecular sciences · 2021Article
- Engineering Bacillus subtilis ATCC 6051a for the production of recombinant catalases.Journal of industrial microbiology & biotechnology · 2021Article
- Improving the Production of Riboflavin by Introducing a Mutant Ribulose 5-Phosphate 3-Epimerase Gene inFrontiers in bioengineering and biotechnology · 2021Article
- Article
- An update of the suicide plasmid-mediated genome editing system in Corynebacterium glutamicum.Microbial biotechnology · 2019Article
- Development and characterization of a CRISPR/Cas9n-based multiplex genome editing system forBiotechnology for biofuels · 2019Article
- Recent advances in CRISPR/Cas9 mediated genome editing in Bacillus subtilis.World journal of microbiology & biotechnology · 2018Review
- T7 RNA polymerase-driven inducible cell lysis for DNA transfer from Escherichia coli to Bacillus subtilis.Microbial biotechnology · 2017Article
- Genetic Tools for the Enhancement of Probiotic Properties.Microbiology spectrum · 2017Review
- Development of a CRISPR-Cas9 Tool Kit for Comprehensive Engineering of Bacillus subtilis.Applied and environmental microbiology · 2016Article
- Characterization of genome-reduced Bacillus subtilis strains and their application for the production of guanosine and thymidine.Microbial cell factories · 2016Article
- Metabolic engineering of Bacillus subtilis for chiral pure meso-2,3-butanediol production.Biotechnology for biofuels · 2016Article
- Combining Genes from Multiple Phages for Improved Cell Lysis and DNA Transfer from Escherichia coli to Bacillus subtilis.PloS one · 2016Article
- Deregulation of purine pathway in Bacillus subtilis and its use in riboflavin biosynthesis.Microbial cell factories · 2014Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Bacillus subtilis has been a model for gram-positive bacteria and it has long been exploited for industrial and biotechnological applications. However, the availability of facile genetic tools for physiological analysis has generally lagged substantially behind traditional genetic models such as Escherichia coli and Saccharomyces cerevisiae. In this work, we have developed an efficient, precise and scarless method for rapid multiple genetic modifications without altering the chromosome of B. subtilis. This method employs upp gene as a counter-selectable marker, double-strand break (DSB) repair caused by exogenous endonuclease I-SceI and comK overexpression for fast preparation of competent cell. Foreign dsDNA can be simply and efficiently integrated into the chromosome by double-crossover homologous recombination. The DSB repair is a potent inducement for stimulating the second intramolecular homologous recombination, which not only enhances the frequency of resolution by one to two orders of magnitude, but also selects for the resolved product. This method has been successfully and reiteratively used in B. subtilis to deliver point mutations, to generate in-frame deletions, and to construct large-scale deletions. Experimental results proved that it allowed repeated use of the selectable marker gene for multiple modifications and could be a useful technique for B. subtilis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.