ArticlePloS one2013
A proteomic investigation of soluble olfactory proteins in Anopheles gambiae.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 42 citations in OpenAlex.
- Data-driven networking of global transcriptomics and male sexual development in the main malaria vector, Anopheles funestus.Scientific reports · 2023Article
- Shotgun and TMT-Labeled Proteomic Analysis of the Ovarian Proteins of an Insect Vector, Aedes aegypti (Diptera: Culicidae).Journal of insect science (Online) · 2022Article
- A Comparative Evaluation of the Structural and Dynamic Properties of Insect Odorant Binding Proteins.Biomolecules · 2022Article
- Linked-read sequencing identifies abundant microinversions and introgression in the arboviral vector Aedes aegypti.BMC biology · 2020Article
- Molecular characteristics of odorant-binding protein 1 in Anopheles maculipennis.Malaria journal · 2020Article
- Functional Analysis of Two Odorant-Binding Proteins, MaltOBP9 and MaltOBP10, inFrontiers in physiology · 2020Article
- Proteomics reveals localization of cuticular proteins in Anopheles gambiae.Insect biochemistry and molecular biology · 2019Article
- Functional Characterization of Odorant Binding Protein 27 (RproOBP27) FromFrontiers in physiology · 2018Article
- Profiles of soluble proteins in chemosensory organs of three members of the afro-tropical Anopheles gambiae complex.Comparative biochemistry and physiology. Part D, Genomics & proteomics · 2017Article
- Distribution of cuticular proteins in different structures of adult Anopheles gambiae.Insect biochemistry and molecular biology · 2016Article
- Brain proteomics of Anopheles gambiae.Omics : a journal of integrative biology · 2014Article
- Soluble proteins of chemical communication: an overview across arthropods.Frontiers in physiology · 2014Review
Corrections and comments
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Authors and funding
14 authors at 5 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Odorant-binding proteins (OBPs) and chemosensory proteins (CSPs) are small soluble polypeptides that bind semiochemicals in the lymph of insect chemosensilla. In the genome of Anopheles gambiae, 66 genes encode OBPs and 8 encode CSPs. Here we monitored their expression through classical proteomics (2D gel-MS analysis) and a shotgun approach. The latter method proved much more sensitive and therefore more suitable for tiny biological samples as mosquitoes antennae and eggs. Females express a larger number and higher quantities of OBPs in their antennae than males (24 vs 19). OBP9 is the most abundant in the antennae of both sexes, as well as in larvae, pupae and eggs. Of the 8 CSPs, 4 were detected in antennae, while SAP3 was the only one expressed in larvae. Our proteomic results are in fairly good agreement with data of RNA expression reported in the literature, except for OBP4 and OBP5, that we could not identify in our analysis, nor could we detect in Western Blot experiments. The relatively limited number of soluble olfactory proteins expressed at relatively high levels in mosquitoes makes further studies on the coding of chemical messages at the OBP level more accessible, providing for few specific targets. Identification of such proteins in Anopheles gambiae might facilitate future studies on host finding behavior in this important disease vector.
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