ArticleInvestigative ophthalmology & visual science2014
Biophysical and morphological evaluation of human normal and dry eye meibum using hot stage polarized light microscopy.
Article in Investigative ophthalmology & visual science, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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Who cites it
36 citing papers in PubMed, 61 citations in OpenAlex.
- Systane iLux Thermal Pulsation System in the Treatment of Meibomian Gland Dysfunction: A Post-Hoc Analysis of a 12-Month, Randomized, Multicenter Study.Clinical ophthalmology (Auckland, N.Z.) · 2022Trial
- A Controlled-Area Droplet Evaporimeter Reveals Surface Pressure-Dependent Evaporation Resistance of the Tear Film Lipid Layer.Investigative ophthalmology & visual science · 2026Article
- Insulin and Insulin-like Growth Factor 1 Signaling as a Modulator of MYC Expression in the Meibomian Gland.Biomedicines · 2026Article
- Detailed composition of wax esters in mouse sebum and the involvement of FAR2 and AWAT2.iScience · 2026Article
- Corneal wound healing in spaceflight: implications of microgravity-induced molecular signaling modulations for corneal health.Eye (London, England) · 2024Article
- Meibomian gland lipid alterations and ocular surface sequela in Awat2 knockout murine model of meibomian gland dysfunction and evaporative dry eye disease.The ocular surface · 2024Article
- Meibography signal intensity as a novel image biomarker for meibomian gland function: evidence from cross-sectional and longitudinal analyses.Quantitative imaging in medicine and surgery · 2024Article
- High Resolution Images of Human Meibum Spread on Saline.Investigative ophthalmology & visual science · 2024Article
- The Yin and Yang of non-immune and immune responses in meibomian gland dysfunction.The ocular surface · 2024Article
- Modeling meibum secretion: Alternatives for obstructive Meibomian Gland Dysfunction (MGD).The ocular surface · 2024Article
- Meibomian Gland Dysfunction Clinical Practice Guidelines.Japanese journal of ophthalmology · 2023Article
- Human Meibum and Tear Film Derived (O-Acyl)-Omega-Hydroxy Fatty Acids as Biomarkers of Tear Film Dynamics in Meibomian Gland Dysfunction and Dry Eye Disease.Investigative ophthalmology & visual science · 2021Article
- Depletion of Cholesteryl Esters Causes Meibomian Gland Dysfunction-Like Symptoms in aInternational journal of molecular sciences · 2021Article
- Physiological effects of inactivation and the roles of Elovl3/ELOVL3 in maintaining ocular homeostasis.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2021Article
- Androgen and meibomian gland dysfunction: from basic molecular biology to clinical applications.International journal of ophthalmology · 2021Review
- Delineating a novel metabolic high triglycerides-low waxes syndrome that affects lipid homeostasis in meibomian and sebaceous glands.Experimental eye research · 2020Article
- Comprehensive profiling of Asian and Caucasian meibomian gland secretions reveals similar lipidomic signatures regardless of ethnicity.Scientific reports · 2020Article
- Effects of sex (or lack thereof) on meibogenesis in mice (Mus musculus): Comparative evaluation of lipidomes and transcriptomes of male and female tarsal plates.The ocular surface · 2019Article
- Structural Differences in Meibum From Donors After Hematopoietic Stem Cell Transplantations.Cornea · 2019Article
- On the pivotal role ofFASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2019Article
Corrections and comments
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Authors and funding
9 authors at 2 institutions in 1 country.
Funding
Abstract
purposeTo study melting characteristics and the morphology of human and mouse meibum.
methodsHot stage cross-polarized light microscopy (HSPM) and immunohistochemical approaches were used.
resultsIsolated human meibum, and meibum of mice (either isolated or within the meibomian ducts of mice), were found to be in liquid-crystal state at physiological temperatures. Melting of both types of meibum started at approximately 10°C and was completed at approximately 40°C. Melting curves of isolated meibum and meibum inside the meibomian ducts were multiphasic with at least two or three clearly defined phase transition temperatures, typically at approximately 12 ± 2°C (minor transition), 21 ± 3°C, and 32 ± 3°C, regardless the source of meibum. Melting was highly cooperative in nature. Samples of abnormal human meibum collected from dry eye patients with meibomian gland dysfunction often showed an increased presence of nonlipid, nonmelting, nonbirefringent, chloroform-insoluble inclusions of a protein nature. The inclusions were positively stained for cytokeratins. The presence of these inclusions was semiquantitatively characterized using a newly proposed 0 to 4 scale. In the presence of large amounts of these inclusions, melting characteristics of meibum and its structural integrity were altered.
conclusionsHSPM is an effective tool that is suitable for biophysical and morphological evaluation of meibum. Morphological properties and melting characteristics of human meibum were found to be similar to those of mice. Abnormal meibum of many dry eye patients contained large quantities of nonlipid, protein-like inclusions, which were routinely absent in meibum of normal controls.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.