ArticleThe Journal of antibiotics2014
Quantitative molecular networking to profile marine cyanobacterial metabolomes.
Article in The Journal of antibiotics, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
29 citing papers in PubMed.
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- A Four-Step Platform to Optimize Growth Conditions for High-Yield Production of Siderophores in Cyanobacteria.Metabolites · 2023Article
- Metabolomics on the study of marine organisms.Metabolomics : Official journal of the Metabolomic Society · 2022Review
- Neurobehavioral alternations of the female offspring born to polycystic ovary syndrome model rats administered by Chinese herbal medicine.Chinese medicine · 2021Article
- Alkaloids in Contemporary Drug Discovery to Meet Global Disease Needs.Molecules (Basel, Switzerland) · 2021Review
- Inducing secondary metabolite production of Aspergillus sydowii through microbial co-culture with Bacillus subtilis.Microbial cell factories · 2021Article
- BiG-FAM: the biosynthetic gene cluster families database.Nucleic acids research · 2021Article
- Lipidomics of Environmental Microbial Communities. II: Characterization Using Molecular Networking and Information Theory.Frontiers in microbiology · 2021Article
- Heterologous Expression of Cryptomaldamide in a Cyanobacterial Host.ACS synthetic biology · 2020Article
- Feature-based molecular networking in the GNPS analysis environment.Nature methods · 2020Article
- Reproducible molecular networking of untargeted mass spectrometry data using GNPS.Nature protocols · 2020Article
- Insights into the Metabolome of the CyanobacteriumMetabolites · 2020Article
- The Marine Natural Product Manzamine A Inhibits Cervical Cancer by Targeting the SIX1 Protein.Journal of natural products · 2020Article
- Integrated Analytical Tools for Accessing Acridones and Unrelated Phenylacrylamides fromMolecules (Basel, Switzerland) · 2019Article
- Discovery of novel glycerolated quinazolinones from Streptomyces sp. MBT27.Journal of industrial microbiology & biotechnology · 2019Article
- Integrated Genomic and Metabolomic Approach to the Discovery of Potential Anti-Quorum Sensing Natural Products from Microbes Associated with Marine Samples from Singapore.Marine drugs · 2019Article
- Ketoreductase Domain Dysfunction Expands Chemodiversity: Malyngamide Biosynthesis in the Cyanobacterium Okeania hirsuta.ACS chemical biology · 2018Article
- Unique amalgamation of primary and secondary structural elements transform peptaibols into potent bioactive cell-penetrating peptides.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Prioritizing Natural Product Diversity in a Collection of 146 Bacterial Strains Based on Growth and Extraction Protocols.Journal of natural products · 2017Article
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
Untargeted liquid chromatography-MS (LC-MS) is used to rapidly profile crude natural product (NP) extracts; however, the quantity of data produced can become difficult to manage. Molecular networking based on MS/MS data visualizes these complex data sets to aid their initial interpretation. Here, we developed an additional visualization step for the molecular networking workflow to provide relative and absolute quantitation of a specific compound in an extract. The new visualization also facilitates combination of several metabolomes into one network, and so was applied to an MS/MS data set from 20 crude extracts of cultured marine cyanobacteria. The resultant network illustrates the high chemical diversity present among marine cyanobacteria. It is also a powerful tool for locating producers of specific metabolites. In order to dereplicate and identify culture-based sources of known compounds, we added MS/MS data from 60 pure NPs and NP analogs to the 20-strain network. This dereplicated six metabolites directly and offered structural information on up to 30 more. Most notably, our visualization technique allowed us to identify and quantitatively compare several producers of the bioactive and biosynthetically intriguing lipopeptide malyngamide C. Our most prolific producer, a Panamanian strain of Okeania hirsuta (PAB10FEB10-01), was found to produce at least 0.024 mg of malyngamide C per mg biomass (2.4%, w/dw) and is now undergoing genome sequencing to access the corresponding biosynthetic machinery.
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