ArticleOncotarget2013
In situ mutation detection and visualization of intratumor heterogeneity for cancer research and diagnostics.
Article in Oncotarget, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
What it found
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Who cites it
29 citing papers in PubMed, 49 citations in OpenAlex.
- Spatial profiling of gene editing by in situ sequencing in mice and macaques.Nature biomedical engineering · 2026Article
- Tumour promotion through the lens of evolution.Nature · 2026Review
- Improving Spatial Transcriptomics with Dual-Color Coding and Cell Painting.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Towards Post-Genomic Oncology: Embracing Cancer Complexity via Artificial Intelligence, Multi-Targeted Therapeutics, Drug Repurposing, and Innovative Study Designs.International journal of molecular sciences · 2025Review
- Predicting Molecular Subtype and Survival of Rhabdomyosarcoma Patients Using Deep Learning of H&E Images: A Report from the Children's Oncology Group.Clinical cancer research : an official journal of the American Association for Cancer Research · 2023Article
- Article
- Circulating tumour cells and circulating cell-free DNA in patients with lung cancer: a comparison between thoracotomy and video-assisted thoracoscopic surgery.BMJ open respiratory research · 2021Article
- Biological significance of KRAS mutant allele expression in ovarian endometriosis.Cancer science · 2021Article
- Generation of in situ sequencing based OncoMaps to spatially resolve gene expression profiles of diagnostic and prognostic markers in breast cancer.EBioMedicine · 2019Article
- Modeling Spatial Correlation of Transcripts with Application to Developing Pancreas.Scientific reports · 2019Article
- Histological processing of un-/cellularized thermosensitive electrospun scaffolds.Histochemistry and cell biology · 2019Article
- Barcode-free next-generation sequencing error validation for ultra-rare variant detection.Nature communications · 2019Article
- Barcoded solid-phase RNA capture for Spatial Transcriptomics profiling in mammalian tissue sections.Nature protocols · 2018Article
- Effects of Microchannel Shape and Ultrasonic Mixing on Microfluidic Padlock Probe Rolling Circle Amplification (RCA) Reactions.Micromachines · 2018Article
- Quantification and localization of oncogenic receptor tyrosine kinase variant transcripts using molecular inversion probes.Scientific reports · 2018Article
- Rare genetic heterogeneity within single tumor discovered for the first time in colorectal liver metastases after liver resection.Oncotarget · 2018Article
- Overview on Clinical Relevance of Intra-Tumor Heterogeneity.Frontiers in medicine · 2018Review
- Visualization of tumor heterogeneity by in situ padlock probe technology in colorectal cancer.Histochemistry and cell biology · 2017Article
- Simultaneous Single-CellJournal of virology · 2017Article
- Precancer Atlas to Drive Precision Prevention Trials.Cancer research · 2017Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
10 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Current assays for somatic mutation analysis are based on extracts from tissue sections that often contain morphologically heterogeneous neoplastic regions with variable contents of normal stromal and inflammatory cells, obscuring the results of the assays. We have developed an RNA-based in situ mutation assay that targets oncogenic mutations in a multiplex fashion that resolves the heterogeneity of the tissue sample. Activating oncogenic mutations are targets for a new generation of cancer drugs. For anti-EGFR therapy prediction, we demonstrate reliable in situ detection of KRAS mutations in codon 12 and 13 in colon and lung cancers in three different types of routinely processed tissue materials. High-throughput screening of KRAS mutation status was successfully performed on a tissue microarray. Moreover, we show how the patterns of expressed mutated and wild-type alleles can be studied in situ in tumors with complex combinations of mutated EGFR, KRAS and TP53. This in situ method holds great promise as a tool to investigate the role of somatic mutations during tumor progression and for prediction of response to targeted therapy.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.