ArticleThe Plant cell2013
Pollen tube growth regulation by free anions depends on the interaction between the anion channel SLAH3 and calcium-dependent protein kinases CPK2 and CPK20.
Article in The Plant cell, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 63 papers.
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Who cites it
63 citing papers in PubMed, 139 citations in OpenAlex.
- Calcium-dependent protein kinase signalling in plant immune responses: current status and future prospects.Planta · 2026Review
- Deciphering the Transcriptomic Dynamics of Self-Incompatibility in Yellow Passion Fruit: Evidence of Modified Sporophytic Mechanism.Plants (Basel, Switzerland) · 2026Article
- SlSLAH2 mediates malate exudation and contributes to aluminum tolerance.Nature communications · 2026Article
- Comprehensive analysis of the LiCPKs in Lagerstroemia indica for insights into evolution and regulatory roles in flower maturation and bud-to-branch development.BMC plant biology · 2026Article
- Calcium-Dependent Protein Kinases in Plants: Structure, Signaling, and Multifaceted Regulatory Roles in Development and Stress Adaptation.International journal of molecular sciences · 2026Review
- OsCML17 a pollen-expressed calmodulin-like protein regulates pollen germination and tube growth in rice (Oryza sativa).Plant cell reports · 2026Article
- Structural insights into the coordinated regulation of the SLAH family in Arabidopsis thaliana.Nature communications · 2025Article
- Identification, characterization and expression analysis of the CDPK family in kelp Saccharina japonica.BMC genomics · 2025Article
- Article
- From gametes to zygote: Mechanistic advances and emerging possibilities in plant reproduction.Plant physiology · 2024Review
- Calmodulin-Domain Protein Kinase PiCDPK1 Interacts with the 14-3-3-like Protein NtGF14 to Modulate Pollen Tube Growth.Plants (Basel, Switzerland) · 2024Article
- Imaging of plant calcium-sensor kinase conformation monitors real time calcium-dependent decoding in planta.The Plant cell · 2024Article
- The putative myristoylome of Physcomitrium patens reveals conserved features of myristoylation in basal land plants.Plant cell reports · 2023Article
- The miR166d/International journal of molecular sciences · 2023Article
- The anion channel SLAH3 interacts with potassium channels to regulate nitrogen-potassium homeostasis and the membrane potential in Arabidopsis.The Plant cell · 2023Article
- Calmodulin Domain Protein Kinase PiCDPK1 Regulates Pollen Tube Growth Polarity through Interaction with RhoGDI.Plants (Basel, Switzerland) · 2022Article
- Cassava (Frontiers in plant science · 2022Article
- Optogenetics for light control of biological systems.Nature reviews. Methods primers · 2022Article
- Molecular Characterization Reveals the Involvement of Calcium Dependent Protein Kinases in Abiotic Stress Signaling and Development in Chickpea (Frontiers in plant science · 2022Article
- Constitutive Active CPK30 Interferes With Root Growth and Endomembrane Trafficking inFrontiers in plant science · 2022Article
3 more citing papers are in PubMed but not listed here.
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Authors and funding
9 authors at 4 institutions in 4 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Apical growth in pollen tubes (PTs) is associated with the presence of tip-focused ion gradients and fluxes, implying polar localization or regulation of the underlying transporters. The molecular identity and regulation of anion transporters in PTs is unknown. Here we report a negative gradient of cytosolic anion concentration focused on the tip, in negative correlation with the cytosolic Ca(2+) concentration. We hypothesized that a possible link between these two ions is based on the presence of Ca(2+)-dependent protein kinases (CPKs). We characterized anion channels and CPK transcripts in PTs and analyzed their localization. Yellow fluorescent protein (YFP) tagging of a homolog of SLOW ANION CHANNEL-ASSOCIATED1 (SLAH3:YFP) was widespread along PTs, but, in accordance with the anion efflux, CPK2/CPK20/CPK17/CPK34:YFP fluorescence was strictly localized at the tip plasma membrane. Expression of SLAH3 with either CPK2 or CPK20 (but not CPK17/CPK34) in Xenopus laevis oocytes elicited S-type anion channel currents. Interaction of SLAH3 with CPK2/CPK20 (but not CPK17/CPK34) was confirmed by Förster-resonance energy transfer fluorescence lifetime microscopy in Arabidopsis thaliana mesophyll protoplasts and bimolecular fluorescence complementation in living PTs. Compared with wild-type PTs, slah3-1 and slah3-2 as well as cpk2-1 cpk20-2 PTs had reduced anion currents. Double mutant cpk2-1 cpk20-2 and slah3-1 PTs had reduced extracellular anion fluxes at the tip. Our studies provide evidence for a Ca(2+)-dependent CPK2/CPK20 regulation of the anion channel SLAH3 to regulate PT growth.
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