ArticleProceedings of the National Academy of Sciences of the United States of America1986
Cloning of cDNA and amino acid sequence of a cytokeratin expressed in oocytes of Xenopus laevis.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1986. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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20 citing papers in PubMed, 93 citations in OpenAlex.
- Mechanical and signaling roles for keratin intermediate filaments in the assembly and morphogenesis ofDevelopment (Cambridge, England) · 2017Article
- A mechanoresponsive cadherin-keratin complex directs polarized protrusive behavior and collective cell migration.Developmental cell · 2012Article
- Structure and functions of keratin proteins in simple, stratified, keratinized and cornified epithelia.Journal of anatomy · 2009Review
- Mechanical properties of Xenopus egg cytoplasmic extracts.Biophysical journal · 2005Article
- A nontetrameric species is the major soluble form of keratin in Xenopus oocytes and rabbit reticulocyte lysates.The Journal of cell biology · 1996Article
- The 47-kD lens-specific protein phakinin is a tailless intermediate filament protein and an assembly partner of filensin.The Journal of cell biology · 1993Article
- Bovine filensin possesses primary and secondary structure similarity to intermediate filament proteins.The Journal of cell biology · 1993Article
- Article
- Evidence that the deep keratin filament systems of the Xenopus embryo act to ensure normal gastrulation.Proceedings of the National Academy of Sciences of the United States of America · 1992Article
- Cytokeratin phosphorylation, cytokeratin filament severing and the solubilization of the maternal mRNA Vg1.The Journal of cell biology · 1991Article
- The coiled coil of in vitro assembled keratin filaments is a heterodimer of type I and II keratins: use of site-specific mutagenesis and recombinant protein expression.The Journal of cell biology · 1990Article
- Epidermal growth factor-induced selective phosphorylation of cultured rat hepatocyte 55-kD cytokeratin before filament reorganization and DNA synthesis.The Journal of cell biology · 1989Article
- An epithelium-type cytoskeleton in a glial cell: astrocytes of amphibian optic nerves contain cytokeratin filaments and are connected by desmosomes.The Journal of cell biology · 1989Article
- The structure and organization of the human heavy neurofilament subunit (NF-H) and the gene encoding it.The EMBO journal · 1988Article
- Identification of domains involved in nuclear uptake and histone binding of protein N1 of Xenopus laevis.The EMBO journal · 1988Article
- Molecular characterization and expression of the stratification-related cytokeratins 4 and 15.The Journal of cell biology · 1988Article
- Concerted gene duplications in the two keratin gene families.Journal of molecular evolution · 1988Article
- Analysis of cytokeratin domains by cloning and expression of intact and deleted polypeptides in Escherichia coli.The EMBO journal · 1987Article
- Identification of the conserved, conformation-dependent cytokeratin epitope recognized by monoclonal antibody (lu-5).Virchows Archiv. A, Pathological anatomy and histopathology · 1987Article
- Molecular characterization of a karyophilic, histone-binding protein: cDNA cloning, amino acid sequence and expression of nuclear protein N1/N2 of Xenopus laevis.The EMBO journal · 1986Article
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2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Using a cDNA clone from the ovary of the frog, Xenopus laevis, we have identified the mRNA and determined the complete amino acid sequence of a major cytoskeletal protein expressed in the oocyte. A comparison with other cytoskeletal proteins of Xenopus and mammals identifies this polypeptide Mr 55,700 as a nonepidermal kind of cytokeratin of the basic (type II) subfamily, which represents the amphibian equivalent to cytokeratin no. 8 of simple epithelia of higher mammals. The sequence data demonstrate the high evolutionary stability of this protein. This cytokeratin and its mRNA are present in oocytes, eggs, embryos, liver, and intestinal mucosa of adult frogs, as well as cultured kidney epithelial cells. We suggest that epithelial cell differentiation in early stages of Xenopus embryogenesis differs from other known pathways of cell differentiation in that major cell-type-specific proteins--i.e., cytokeratins of the simple epithelial type--and their mRNAs are maternally provided and distributed to early epithelial cells by special sorting mechanisms.
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