ArticleEpigenetics & chromatin2013
Transcription through enhancers suppresses their activity in Drosophila.
Article in Epigenetics & chromatin, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 34 citations in OpenAlex.
- Enhancer RNA transcription near segmentation gene enhancers can be analyzed in situ using FISH.G3 (Bethesda, Md.) · 2026Article
- Transcriptional Readthrough Interrupts Boundary Function in Drosophila.International journal of molecular sciences · 2023Article
- Crol contributes to PRE-mediated repression and Polycomb group proteins recruitment in Drosophila.Nucleic acids research · 2023Article
- Transcriptional read through interrupts boundary function in Drosophila.bioRxiv : the preprint server for biology · 2023Article
- Comparative interactome analysis of the PRE DNA-binding factors: purification of the Combgap-, Zeste-, Psq-, and Adf1-associated proteins.Cellular and molecular life sciences : CMLS · 2022Article
- Boundaries potentiate polycomb response element-mediated silencing.BMC biology · 2021Article
- An insulator blocks access to enhancers by an illegitimate promoter, preventing repression by transcriptional interference.PLoS genetics · 2021Article
- Enhancer occlusion transcripts regulate the activity of human enhancer domains via transcriptional interference: a computational perspective.Nucleic acids research · 2020Article
- Transcription termination sequences support the expression of transgene product secreted with milk.Transgenic research · 2019Article
- A Novel PRE-Element from Drosophila virilis Genome as a Useful Model Silencer.Doklady. Biochemistry and biophysics · 2019Article
- Effect of Transcription on the white Gene Enhancer Integrated into the Intron.Doklady. Biochemistry and biophysics · 2019Article
- Transcriptional read-through is not sufficient to induce an epigenetic switch in the silencing activity of Polycomb response elements.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Eukaryotic enhancers: common features, regulation, and participation in diseases.Cellular and molecular life sciences : CMLS · 2015Review
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- SV40 transcription terminators stabilize the activity of regulatory elements in Drosophila melanogaster.Doklady. Biochemistry and biophysics · 2015Article
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Corrections and comments
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundEnhancer elements determine the level of target gene transcription in a tissue-specific manner, providing for individual patterns of gene expression in different cells. Knowledge of the mechanisms controlling enhancer action is crucial for understanding global regulation of transcription. In particular, enhancers are often localized within transcribed regions of the genome. A number of experiments suggest that transcription can have both positive and negative effects on regulatory elements. In this study, we performed direct tests for the effect of transcription on enhancer activity.
resultsUsing a transgenic reporter system, we investigated the relationship between the presence of pass-through transcription and the activity of Drosophila enhancers controlling the expression of the white and yellow genes. The results show that transcription from different promoters affects the activity of enhancers, counteracting their ability to activate the target genes. As expected, the presence of a transcriptional terminator between the inhibiting promoter and the affected enhancer strongly reduces the suppression. Moreover, transcription leads to dislodging of the Zeste protein that is responsible for the enhancer-dependent regulation of the white gene, suggesting a 'transcription interference' mechanism for this regulation.
conclusionsOur findings suggest a role for pass-through transcription in negative regulation of enhancer activity.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.