ArticlePloS one2013
Leishmania amazonensis promastigotes present two distinct modes of nucleus and kinetoplast segregation during cell cycle.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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15 citing papers in PubMed, 125 citations in OpenAlex.
- Cell cycle ofCurrent zoology · 2026Article
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- Leishmania guyanensis M4147 as a new LRV1-bearing model parasite: Phosphatidate phosphatase 2-like protein controls cell cycle progression and intracellular lipid content.PLoS neglected tropical diseases · 2022Article
- Synchronization of Leishmania amazonensis Cell Cycle Using Hydroxyurea.Methods in molecular biology (Clifton, N.J.) · 2022Article
- The Trypanosomatids Cell Cycle: A Brief Report.Methods in molecular biology (Clifton, N.J.) · 2022Review
- Cell Cycle, Telomeres, and Telomerase inCells · 2021Review
- Comparative Analysis of the Minimum Number of Replication Origins in Trypanosomatids and Yeasts.Genes · 2020Article
- Transcription activity contributes to the firing of non-constitutive origins in African trypanosomes helping to maintain robustness in S-phase duration.Scientific reports · 2019Article
- Cell cycle and cleavage events during in vitro cultivation of bloodstream forms of Trypanosoma lewisi, a zoonotic pathogen.Cell cycle (Georgetown, Tex.) · 2019Article
- Leishmanicidal Activity of Isoselenocyanate Derivatives.Antimicrobial agents and chemotherapy · 2019Article
- Analysis of DNA Exchange Using Thymidine Analogs (ADExTA) inBio-protocol · 2018Article
- MiR-3188 Inhibits Non-small Cell Lung Cancer Cell Proliferation Through FOXO1-Mediated mTOR-p-PI3K/AKT-c-JUN Signaling Pathway.Frontiers in pharmacology · 2018Article
- KHARON Is an Essential Cytoskeletal Protein Involved in the Trafficking of Flagellar Membrane Proteins and Cell Division in African Trypanosomes.The Journal of biological chemistry · 2016Article
- In Vitro and in Vivo Activity of Multitarget Inhibitors against Trypanosoma brucei.ACS infectious diseases · 2015Article
- N-butyl-[1-(4-methoxy)phenyl-9H-β-carboline]-3-carboxamide prevents cytokinesis in Leishmania amazonensis.Antimicrobial agents and chemotherapy · 2014Article
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Authors and funding
8 authors at 5 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Here, we show the morphological events associated with organelle segregation and their timing in the cell cycle of a reference strain of Leishmania (L.) amazonensis promastigotes, the main causative agent of Tegumentary leishmaniasis in the Americas. We show evidences that during the cell cycle, L. amazonensis promastigotes present two distinct modes of nucleus and kinetoplast segregation, which occur in different temporal order in different proportions of cells. We used DAPI-staining and EdU-labeling to monitor the segregation of DNA-containing organelles and DNA replication in wild-type parasites. The emergence of a new flagellum was observed using a specific monoclonal antibody. The results show that L. amazonensis cell cycle division is peculiar, with 65% of the dividing cells duplicating the kinetoplast before the nucleus, and the remaining 35% doing the opposite or duplicating both organelles concomitantly. In both cases, the new flagellum appeared during S to G2 phase in 1N1K cells and thus before the segregation of both DNA-containing organelles; however, we could not determine the exact timing of flagellar synthesis. Most of these results were confirmed by the synchronization of parasites using hydroxyurea. Altogether, our data show that during the cell cycle of L. amazonensis promastigotes, similarly to L. donovani, the segregation of nucleus and kinetoplast do not follow a specific order, especially when compared to other trypanosomatids, reinforcing the idea that this characteristic seems to be species-specific and may represent differences in cellular biology among members of the Leishmania genus.
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