ArticlePloS one2013
Investigating the allosteric regulation of YfiN from Pseudomonas aeruginosa: clues from the structure of the catalytic domain.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed.
- The versatility of the CHASE domain in one-component systems of c-di-GMP signal transduction fromJournal of bacteriology · 2026Article
- Cyclic-di-GMP signalling mutants drive ecological succession and self-generated diversity in experimentally evolved biofilms ofMicrobiology (Reading, England) · 2025Article
- Exploring Innovative Approaches to Isolate a One-Component c-di-GMP Transducer: A Pilot Study.Advances in experimental medicine and biology · 2025Article
- Article
- Characterization of a soluble library of themicroLife · 2023Article
- Identification of Cyclic-di-GMP-Modulating Protein Residues by Bidirectionally Evolving a Social Behavior in Pseudomonas fluorescens.mSystems · 2022Article
- Pseudomonas aeruginosa Strains from Both Clinical and Environmental Origins Readily Adopt a Stable Small-Colony-Variant Phenotype Resulting from Single Mutations in c-di-GMP Pathways.Journal of bacteriology · 2022Article
- Biofilm Maintenance as an Active Process: Evidence that Biofilms Work Hard to Stay Put.Journal of bacteriology · 2022Review
- YfiB: An Outer Membrane Protein Involved in the Virulence ofMicroorganisms · 2022Article
- The Diguanylate Cyclase YfiN of Pseudomonas aeruginosa Regulates Biofilm Maintenance in Response to Peroxide.Journal of bacteriology · 2022Article
- Bacteriophage-mediated interference of the c-di-GMP signalling pathway in Pseudomonas aeruginosa.Microbial biotechnology · 2021Article
- Studying GGDEF Domain in the Act: Minimize Conformational Frustration to Prevent Artefacts.Life (Basel, Switzerland) · 2021Article
- Article
- Characterization and analysis of a novel diguanylate cyclase PA0847 fromInfection and drug resistance · 2019Article
- A bipartite periplasmic receptor-diguanylate cyclase pair (XAC2383-XAC2382) in the bacteriumThe Journal of biological chemistry · 2018Article
- Site-specific His/Asp phosphoproteomic analysis of prokaryotes reveals putative targets for drug resistance.BMC microbiology · 2017Article
- Versatile modes of cellular regulation via cyclic dinucleotides.Nature chemical biology · 2017Review
- Article
- Structural insights into the regulatory mechanism of the Pseudomonas aeruginosa YfiBNR system.Protein & cell · 2016Article
- In Silico Discovery and In Vitro Validation of Catechol-Containing Sulfonohydrazide Compounds as Potent Inhibitors of the Diguanylate Cyclase PleD.Journal of bacteriology · 2016Article
Corrections and comments
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Pseudomonas aeruginosa is responsible for a plethora of biofilm mediated chronic infections among which cystic fibrosis pneumonia is the most frightening. The long-term survival strategy of P. aeruginosa in the patients lungs is based on a fine balance of virulence vs dormant states and on genetic adaptation, in order to select persistent phenotypes as the small colony variants (SCVs), which strongly correlate with antibiotic resistance and poor lung function. Recent studies have coupled SCV with increased levels of the signaling molecule cyclic di-GMP, and demonstrated the central role of the diguanylate cyclase YfiN, part of the tripartite signaling module YifBNR, in c-di-GMP dependent SCV regulation. YfiN, also called TpbB, is a multi-domain membrane enzyme connecting periplasmic stimuli to cytosolic c-di-GMP production by an allosteric inside-out signaling mechanism that, due to the lack of structural data, is still largely hypothetical. We have solved the crystal structure of the catalytic domain (GGDEF), and measured the enzymatic activity of the cytosolic portion in real-time by means of a newly developed method. Based on these results we demonstrate that, unlike other diguanylate cyclase, YfiN does not undergo product feedback inhibition, and that the presence of the HAMP domain is required for dimerization and catalysis. Coupling our structural and kinetic data with an in silico study we are now able to propose a model for the allosteric regulation of YfiN.
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