ArticlePloS one2013
Hybridization-based detection of Helicobacter pylori at human body temperature using advanced locked nucleic acid (LNA) probes.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
18 citing papers in PubMed, 45 citations in OpenAlex.
- Dynamics of the oral microbiome during orthodontic treatment and antimicrobial advances for orthodontic appliances.iScience · 2024Review
- Imaging biofilms using fluorescenceFrontiers in cellular and infection microbiology · 2023Review
- Liposome Delivery of Nucleic Acids in Bacteria: TowardACS infectious diseases · 2022Article
- Spectral imaging and nucleic acid mimics fluorescenceFrontiers in microbiology · 2022Article
- Can Vitamin B12 Assist the Internalization of Antisense LNA Oligonucleotides into Bacteria?Antibiotics (Basel, Switzerland) · 2021Article
- FISH Variants.Methods in molecular biology (Clifton, N.J.) · 2021Review
- Application of Nucleic Acid Mimics in Fluorescence In Situ Hybridization.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Bioinformatic Tools and Guidelines for the Design of Fluorescence In Situ Hybridization Probes.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Optimizing locked nucleic acid/2'-O-methyl-RNA fluorescence in situ hybridization (LNA/2'OMe-FISH) procedure for bacterial detection.PloS one · 2019Article
- A self-assembled, modular nucleic acid-based nanoscaffold for multivalent theranostic medicine.Theranostics · 2019Article
- Identification of pathogenic bacteria in complex samples using a smartphone based fluorescence microscope.RSC advances · 2018Article
- Targeting miR-9 in gastric cancer cells using locked nucleic acid oligonucleotides.BMC molecular biology · 2018Article
- Current techniques for visualizing RNA in cells.F1000Research · 2016Review
- Fluorescence In Vivo Hybridization (FIVH) for Detection of Helicobacter pylori Infection in a C57BL/6 Mouse Model.PloS one · 2016Article
- Effect of Native Gastric Mucus on in vivo Hybridization Therapies Directed at Helicobacter pylori.Molecular therapy. Nucleic acids · 2015Article
- Towards Fluorescence In Vivo Hybridization (FIVH) Detection of H. pylori in Gastric Mucosa Using Advanced LNA Probes.PloS one · 2015Article
- Diagnosis of Helicobacter pylori: what should be the gold standard?World journal of gastroenterology · 2014Review
- Biofilms and Helicobacter pylori: Dissemination and persistence within the environment and host.World journal of gastrointestinal pathophysiology · 2014Review
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The understanding of the human microbiome and its influence upon human life has long been a subject of study. Hence, methods that allow the direct detection and visualization of microorganisms and microbial consortia (e.g. biofilms) within the human body would be invaluable. In here, we assessed the possibility of developing a variant of fluorescence in situ hybridization (FISH), named fluorescence in vivo hybridization (FIVH), for the detection of Helicobacter pylori. Using oligonucleotide variations comprising locked nucleic acids (LNA) and 2'-O-methyl RNAs (2'OMe) with two types of backbone linkages (phosphate or phosphorothioate), we were able to successfully identify two probes that hybridize at 37 °C with high specificity and sensitivity for H. pylori, both in pure cultures and in gastric biopsies. Furthermore, the use of this type of probes implied that toxic compounds typically used in FISH were either found to be unnecessary or could be replaced by a non-toxic substitute. We show here for the first time that the use of advanced LNA probes in FIVH conditions provides an accurate, simple and fast method for H. pylori detection and location, which could be used in the future for potential in vivo applications either for this microorganism or for others.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.