ArticlePloS one2013
The role of pH fronts in tissue electroporation based treatments.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
25 citing papers in PubMed.
- Noninvasive Monitoring of Skin pH Changes Induced by Microneedle Electroporation.Sensors and actuators. B, Chemical · 2026Article
- Harnessing the Electrochemical Effects of Electroporation-Based Therapies to Enhance Anti-tumor Immune Responses.Annals of biomedical engineering · 2024Article
- Determination of the Impact of High-Intensity Pulsed Electromagnetic Fields on the Release of Damage-Associated Molecular Pattern Molecules.International journal of molecular sciences · 2023Article
- Effect of Experimental Electrical and Biological Parameters on Gene Transfer by Electroporation: A Systematic Review and Meta-Analysis.Pharmaceutics · 2022Review
- Numerical mesoscale tissue model of electrochemotherapy in liver based on histological findings.Scientific reports · 2022Article
- Electrochemotherapy using thin-needle electrode improves recovery in feline nasal planum squamous cell carcinoma - a translational model.Cancer drug resistance (Alhambra, Calif.) · 2022Article
- The impact of impaired DNA mobility on gene electrotransfer efficiency: analysis in 3D model.Biomedical engineering online · 2021Article
- Inactivation of Bacteria Using Bioactive Nanoparticles and Alternating Magnetic Fields.Nanomaterials (Basel, Switzerland) · 2021Article
- Electroporation as the Immunotherapy Strategy for Cancer in Veterinary Medicine: State of the Art in Latin America.Vaccines · 2020Review
- Article
- Toward a clinical real time tissue ablation technology: combining electroporation and electrolysis (E2).PeerJ · 2020Article
- Electroporation optimizes the uptake of boron-10 by tumor for boron neutron capture therapy (BNCT) mediated by GB-10: a boron biodistribution study in the hamster cheek pouch oral cancer model.Radiation and environmental biophysics · 2019Article
- Ultrasonographic changes in the liver tumors as indicators of adequate tumor coverage with electric field for effective electrochemotherapy.Radiology and oncology · 2018Article
- Simultaneous electroporation and dielectrophoresis in non-electrolytic micro/nano-electroporation.Scientific reports · 2018Article
- ESOPE-Equivalent Pulsing Protocols for Calcium Electroporation: An In Vitro Optimization Study on 2 Cancer Cell Models.Technology in cancer research & treatment · 2018Article
- Article
- Electrosensitization assists cell ablation by nanosecond pulsed electric field in 3D cultures.Scientific reports · 2016Article
- Gene Electrotransfer: A Mechanistic Perspective.Current gene therapy · 2016Review
- Electroporation on microchips: the harmful effects of pH changes and scaling down.Scientific reports · 2015Article
- The feasibility of using irreversible electroporation to introduce pores in bacterial cellulose scaffolds for tissue engineering.Applied microbiology and biotechnology · 2015Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Treatments based on electroporation (EP) induce the formation of pores in cell membranes due to the application of pulsed electric fields. We present experimental evidence of the existence of pH fronts emerging from both electrodes during treatments based on tissue EP, for conditions found in many studies, and that these fronts are immediate and substantial. pH fronts are indirectly measured through the evanescence time (ET), defined as the time required for the tissue buffer to neutralize them. The ET was measured through a pH indicator imaged at a series of time intervals using a four-cluster hard fuzzy-c-means algorithm to segment pixels corresponding to the pH indicator at every frame. The ET was calculated as the time during which the number of pixels was 10% of those in the initial frame. While in EP-based treatments such as reversible (ECT) and irreversible electroporation (IRE) the ET is very short (though enough to cause minor injuries) due to electric pulse characteristics and biological buffers present in the tissue, in gene electrotransfer (GET), ET is much longer, enough to denaturate plasmids and produce cell damage. When any of the electric pulse parameters is doubled or tripled the ET grows and, remarkably, when any of the pulse parameters in GET is halved, the ET drops significantly. Reducing pH fronts has relevant implications for GET treatment efficiency, due to a substantial reduction of plasmid damage and cell loss.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.