ArticlePloS one2013
Potential human pathogenic bacteria in a mixed urban watershed as revealed by pyrosequencing.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed.
- Assessing Recreational Safety of Urban Marine Waters: Long-Term Coliform and Enterococcus Analysis.Water environment research : a research publication of the Water Environment Federation · 2026Article
- Foot Traffic Driven Anthropogenic Activity Alters Phyllosphere Microbial Community Characteristics and Putative Pathogens in Subtropical Urban Green Spaces.Microorganisms · 2025Article
- Ecological Roles and Shared Microbes Differentiate the Plastisphere from Natural Particle-Associated Microbiomes in Urban Rivers.Environmental science & technology · 2025Article
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- A narrative review of wastewater surveillance: pathogens of concern, applications, detection methods, and challenges.Frontiers in public health · 2024Review
- Microbial Community Analysis and Food Safety Practice Survey-Based Hazard Identification and Risk Assessment for Controlled Environment Hydroponic/Aquaponic Farming Systems.Frontiers in microbiology · 2022Article
- Seasonal and Technological Shifts of the WHO Priority Multi-Resistant Pathogens in Municipal Wastewater Treatment Plant and Its Receiving Surface Water: A Case Study.International journal of environmental research and public health · 2021Article
- Microplastics with adsorbed contaminants: Mechanisms and Treatment.Environmental challenges (Amsterdam, Netherlands) · 2021Review
- Antimicrobial sensitivity profiling of bacterial communities recovered from effluents of municipal solid waste dumping site.3 Biotech · 2021Article
- Emerging priorities for microbial metagenome research.Bioresource technology reports · 2020Review
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- Amplicon sequencing and imputed metagenomic analysis of waste soil and sediment microbiome reveals unique bacterial communities and their functional attributes.Environmental monitoring and assessment · 2019Article
- Profiling Bacterial Diversity and Potential Pathogens in Wastewater Treatment Plants Using High-Throughput Sequencing Analysis.Microorganisms · 2019Article
- Patterns of Bird-Bacteria Associations.EcoHealth · 2018Review
- Drylands soil bacterial community is affected by land use change and different irrigation practices in the Mezquital Valley, Mexico.Scientific reports · 2018Article
- Aquatic Bacterial Communities Associated With Land Use and Environmental Factors in Agricultural Landscapes Using a Metabarcoding Approach.Frontiers in microbiology · 2018Article
- Comparison of Database Search Methods for the Detection ofFrontiers in microbiology · 2018Article
- Assessment of Fecal Indicator Bacteria and Potential Pathogen Co-Occurrence at a Shellfish Growing Area.Frontiers in microbiology · 2018Article
- Potential human pathogenic bacteria in five hot springs in Eritrea revealed by next generation sequencing.PloS one · 2018Article
- Metagenomic analysis of soil and freshwater from zoo agricultural area with organic fertilization.PloS one · 2017Article
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Current microbial source tracking (MST) methods for water depend on testing for fecal indicator bacterial counts or specific marker gene sequences to identify fecal contamination where potential human pathogenic bacteria could be present. In this study, we applied 454 high-throughput pyrosequencing to identify bacterial pathogen DNA sequences, including those not traditionally monitored by MST and correlated their abundances to specific sources of contamination such as urban runoff and agricultural runoff from concentrated animal feeding operations (CAFOs), recreation park area, waste-water treatment plants, and natural sites with little or no human activities. Samples for pyrosequencing were surface water, and sediment collected from 19 sites. A total of 12,959 16S rRNA gene sequences with average length of ≤400 bp were obtained, and were assigned to corresponding taxonomic ranks using ribosomal database project (RDP), Classifier and Greengenes databases. The percent of total potential pathogens were highest in urban runoff water (7.94%), agricultural runoff sediment (6.52%), and Prado Park sediment (6.00%), respectively. Although the numbers of DNA sequence tags from pyrosequencing were very high for the natural site, corresponding percent potential pathogens were very low (3.78-4.08%). Most of the potential pathogenic bacterial sequences identified were from three major phyla, namely, Proteobacteria, Bacteroidetes, and Firmicutes. The use of deep sequencing may provide improved and faster methods for the identification of pathogen sources in most watersheds so that better risk assessment methods may be developed to enhance public health.
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