ArticlePloS one2013
Global analysis of differentially expressed genes and proteins in the wheat callus infected by Agrobacterium tumefaciens.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 30 citations in OpenAlex.
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- Transcriptional Changes in Damask Rose Suspension Cell Culture Revealed by RNA Sequencing.Plants (Basel, Switzerland) · 2024Article
- Proteome-Level Investigation ofInternational journal of molecular sciences · 2023Article
- Comprehensive Transcriptomic and Metabolic Profiling ofInternational journal of molecular sciences · 2023Article
- Comparative transcriptome analysis reveals compatible and recalcitrant genotypic response of barley microspore-derived embryogenic callus toward Agrobacterium infection.BMC plant biology · 2021Article
- Down-expression of TaPIN1s Increases the Tiller Number and Grain Yield in Wheat.BMC plant biology · 2021Article
- Article
- Overexpression of VIRE2-INTERACTING PROTEIN2 in Arabidopsis regulates genes involved in Agrobacterium-mediated plant transformation and abiotic stresses.Scientific reports · 2019Article
- Genome-wide analysis of differentially expressed genes and the modulation of PEDV infection in Vero E6 cells.Microbial pathogenesis · 2018Article
- The rolB plant oncogene affects multiple signaling protein modules related to hormone signaling and plant defense.Scientific reports · 2018Article
- Genetic analysis of phytoene synthase 1 (Psy1) gene function and regulation in common wheat.BMC plant biology · 2016Article
- Improvement of soybean transformation via Agrobacterium tumefaciens methods involving α-aminooxyacetic acid and sonication treatments enlightened by gene expression profile analysis.Plant cell reports · 2016Article
- Proteomic analysis of the defense response of wheat to the powdery mildew fungus, Blumeria graminis f. sp. tritici.The protein journal · 2014Article
- Identification of proteasome subunit beta type 3 involved in the potential mechanism of corticosteroid protective effectiveness on beta-2 adrenoceptor desensitization by a proteomics approach.Journal of thoracic disease · 2013Article
- LowIn vitro cellular & developmental biology. Plant : journal of the Tissue Culture AssociationArticle
Corrections and comments
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Authors and funding
10 authors at 5 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Agrobacterium-mediated plant transformation is an extremely complex and evolved process involving genetic determinants of both the bacteria and the host plant cells. However, the mechanism of the determinants remains obscure, especially in some cereal crops such as wheat, which is recalcitrant for Agrobacterium-mediated transformation. In this study, differentially expressed genes (DEGs) and differentially expressed proteins (DEPs) were analyzed in wheat callus cells co-cultured with Agrobacterium by using RNA sequencing (RNA-seq) and two-dimensional electrophoresis (2-DE) in conjunction with mass spectrometry (MS). A set of 4,889 DEGs and 90 DEPs were identified, respectively. Most of them are related to metabolism, chromatin assembly or disassembly and immune defense. After comparative analysis, 24 of the 90 DEPs were detected in RNA-seq and proteomics datasets simultaneously. In addition, real-time RT-PCR experiments were performed to check the differential expression of the 24 genes, and the results were consistent with the RNA-seq data. According to gene ontology (GO) analysis, we found that a big part of these differentially expressed genes were related to the process of stress or immunity response. Several putative determinants and candidate effectors responsive to Agrobacterium mediated transformation of wheat cells were discussed. We speculate that some of these genes are possibly related to Agrobacterium infection. Our results will help to understand the interaction between Agrobacterium and host cells, and may facilitate developing efficient transformation strategies in cereal crops.
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