ArticleGenome research2014
A mobile insulator system to detect and disrupt cis-regulatory landscapes in vertebrates.
Article in Genome research, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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Who cites it
6 citing papers in PubMed.
- The landscape of transcription factor promoter activity during vegetative development in Marchantia.The Plant cell · 2024Article
- Multidimensional chromatin profiling of zebrafish pancreas to uncover and investigate disease-relevant enhancers.Nature communications · 2022Article
- Trap-TRAP, a Versatile Tool for Tissue-Specific Translatomics in Zebrafish.Frontiers in cell and developmental biology · 2021Article
- A Conserved Notochord Enhancer Controls Pancreas Development in Vertebrates.Cell reports · 2020Article
- Controlling gene activation by enhancers through a drug-inducible topological insulator.eLife · 2020Article
- MEPD: medaka expression pattern database, genes and more.Nucleic acids research · 2016Article
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Authors and funding
10 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In multicellular organisms, cis-regulation controls gene expression in space and time. Despite the essential implication of cis-regulation in the development and evolution of organisms and in human diseases, our knowledge about regulatory sequences largely derives from analyzing their activity individually and outside their genomic context. Indeed, the contribution of these sequences to the expression of their target genes in their genomic context is still largely unknown. Here we present a novel genetic screen designed to visualize and interrupt gene regulatory landscapes in vertebrates. In this screen, based on the random insertion of an engineered Tol2 transposon carrying a strong insulator separating two fluorescent reporter genes, we isolated hundreds of zebrafish lines containing insertions that disrupt the cis-regulation of tissue-specific expressed genes. We therefore provide a new easy-to-handle tool that will help to disrupt and chart the regulatory activity spread through the vast noncoding regions of the vertebrate genome.
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Registered trials
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