ArticlePlant molecular biology1987
The isolation, characterization and sequence of two divergent β-tubulin genes from soybean (Glycine max L.).
Article in Plant molecular biology, 1987. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 63 citations in OpenAlex.
- Tubulin isotypes optimize distinct spindle positioning mechanisms during yeast mitosis.The Journal of cell biology · 2021Article
- Development of PSP1, a Biostimulant Based on the Elicitor AsES for Disease Management in Monocot and Dicot Crops.Frontiers in plant science · 2018Article
- Laser capture microdissection (LCM) and expression analyses of Glycine max (soybean) syncytium containing root regions formed by the plant pathogen Heterodera glycines (soybean cyst nematode).Plant molecular biology · 2005Article
- Article
- Molecular characterization of the cotton GhTUB1 gene that is preferentially expressed in fiber.Plant physiology · 2002Article
- Differential mRNA degradation of two beta-tubulin isoforms correlates with cytosolic Ca2+ changes in glucan-elicited soybean cells.Plant physiology · 2001Article
- Brassinolides promote the expression of a new Cicer arietinum beta-tubulin gene involved in the epicotyl elongation.Plant molecular biology · 1998Article
- Developmental expression and regulation by light of two closely related beta-tubulin genes in Lupinus albus.Plant molecular biology · 1996Article
- The Pisum sativum TubA1 gene, a member of a small family of alpha-tubulin sequences.Plant molecular biology · 1995Article
- Isolation and characterization of two beta-tubulin cDNA clones from rice.Plant molecular biology · 1994Article
- Characterisation of the cDNA clones of two beta-tubulin genes and their expression in the potato plant (Solanum tuberosum L.).Plant molecular biology · 1994Article
- Characterization of four new beta-tubulin genes and their expression during male flower development in maize (Zea mays L.).Plant molecular biology · 1994Article
- Molecular cloning and sequencing analysis of a beta-tubulin gene from Lupinus albus.Plant molecular biology · 1993Article
- Mapping of beta-tubulin genomic sequences in hexaploid oat (Arena sativa L.).TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 1993Article
- A single amino-acid substitution in the beta-tubulin gene of Neurospora confers both carbendazim resistance and diethofencarb sensitivity.Current genetics · 1992Article
- The beta-tubulin gene family of pea: primary structures, genomic organization and intron-dependent evolution of genes.Plant molecular biology · 1992Article
- Water deficit modulates gene expression in growing zones of soybean seedlings. Analysis of differentially expressed cDNAs, a new beta-tubulin gene, and expression of genes encoding cell wall proteins.Plant molecular biology · 1991Article
- Limited expression of a diverged beta-tubulin gene during soybean (Glycine max [L.] Merr.) development.Plant molecular biology · 1991Article
- The beta-tubulin gene family in Zea mays: two differentially expressed beta-tubulin genes.Plant molecular biology · 1990Article
- Molecular basis for determining the sensitivity of eucaryotes to the antimitotic drug rhizoxin.Molecular & general genetics : MGG · 1990Article
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Two divergent β-tubulin genes (designated Sβ-1 and Sβ-2) were isolated by screening a soybean genomic library with a Chlamydomonas reinhardtii β-tubulin cDNA probe. Restriction fragment analysis of the clones recovered, and of soybean genomic DNA, indicated that these represent two unique classes of structurally different β-tubulin genes in the soybean genome. However, it is possible that unidentified members of these classes or additional highly divergent classes of β-tubulin genes (thus far undetected) exist in the soybean genome. The Sβ-1 and Sβ-2 genomic clones were sequenced, revealing that both are potentially functional genes which would encode β-tubulins of 445 and 449 amino acids, respectively. A comparison of their derived amino acid sequences with β-tubulins from several organisms showed that they are most homologous to Chlamydomonas β-tubulin (85-87%), with lesser degrees of homology to β-tubulins of vertebrate species (79-83%), Trypanosoma brucei (80-81%) and Saccharomyces cerevisiae (66-68%). The amino acid sequences of Sβ-1 and Sβ-2 are as divergent from each other as they are from the Chlamydomonas β-tubulin. The amino acids at the diverged positions in Sβ-2 are nearly all conservative substitutions while in Sβ-1, 18 of the 69 substitutions were non-conservative. Both soybean β-tubulin genes contain two introns in exactly the same positions. The first soybean intron is located in the same position as the third intron of the Chlamydomonas β-tubulin genes. Codon usage in the two soybean β-tubulins is remarkably similar (D (2)=0.87), but differs from codon usage in other soybean genes.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.