ArticleEnvironmental toxicology2015
Up-regulation of Gadd45α after exposure to metal nanoparticles: the role of hypoxia inducible factor 1α.
Article in Environmental toxicology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It has been retracted, and should not be counted. Cited by 13 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
13 citing papers in PubMed, 29 citations in OpenAlex.
- EXPRESSION OF CONCERN: "Up-Regulation of Gadd45α After Exposure to Metal Nanoparticles: The Role of Hypoxia Inducible Factor 1α".Environmental toxicology · 2026Article
- Growth arrest and DNA damage-inducible 45: a new player on inflammatory diseases.Frontiers in immunology · 2025Review
- HMGB1 derived from lung epithelial cells after cobalt nanoparticle exposure promotes the activation of lung fibroblasts.Nanotoxicology · 2024Article
- Nickel nanoparticles induce autophagy and apoptosis via HIF-1α/mTOR signaling in human bronchial epithelial cells.Environmental pollution (Barking, Essex : 1987) · 2023Article
- Comparative synthesis and characterization of nanocomposites using chemical and green approaches including a comparison study onNanoscale advances · 2023Article
- A protective role of autophagy in fine airborne particulate matter-induced apoptosis in LN-229 cells.Toxicology · 2022Article
- Nickel nanoparticle-induced cell transformation: involvement of DNA damage and DNA repair defect through HIF-1α/miR-210/Rad52 pathway.Journal of nanobiotechnology · 2021Article
- Safety assessment of titanium dioxide (E171) as a food additive.EFSA journal. European Food Safety Authority · 2021Article
- The role of miR-21 in nickel nanoparticle-induced MMP-2 and MMP-9 production in mouse primary monocytes: In vitro and in vivo studies.Environmental pollution (Barking, Essex : 1987) · 2020Article
- miR-21 mediates nickel nanoparticle-induced pulmonary injury and fibrosis.Nanotoxicology · 2020Article
- Synthesis and characterization of silver nanoparticle-decorated cobalt nanocomposites (Co@AgNPs) and their density-dependent antibacterial activity.Royal Society open science · 2019Article
- Metallic nanoparticles reduce the migration of human fibroblasts in vitro.Nanoscale research letters · 2017Article
- Cobalt nanoparticles induce lung injury, DNA damage and mutations in mice.Particle and fibre toxicology · 2017Article
Corrections and comments
- Expression of concern · 2026-06-29Concerns/Issues about Image · Duplication of/in Image · Investigation by Journal/Publisher · Investigation by Third Party · Original Data and/or Images not Provided and/or not Available · · see also: https://pubpeer.com/publications/F536C0C265F2DF28F8A4C27C10DA74
- Expression of concern
Authors and funding
9 authors at 3 institutions in 2 countries.
Funding
Abstract
The increased development and use of nanoparticles in various fields may lead to increased exposure, directly affecting human health. Our current knowledge of the health effects of metal nanoparticles such as cobalt and titanium dioxide (Nano-Co and Nano-TiO2 ) is limited but suggests that some metal nanoparticles may cause genotoxic effects including cell cycle arrest, DNA damage, and apoptosis. The growth arrest and DNA damage-inducible 45α protein (Gadd45α) has been characterized as one of the key players in the cellular responses to a variety of DNA damaging agents. The aim of this study was to investigate the alteration of Gadd45α expression in mouse embryo fibroblasts (PW) exposed to metal nanoparticles and the possible mechanisms. Non-toxic doses of Nano-Co and Nano-TiO2 were selected to treat cells. Our results showed that Nano-Co caused a dose- and time-dependent increase in Gadd45α expression, but Nano-TiO2 did not. To investigate the potential pathways involved in Nano-Co-induced Gadd45α up-regulation, we measured the expression of hypoxia inducible factor 1α (HIF-1α) in PW cells exposed to Nano-Co and Nano-TiO2 . Our results showed that exposure to Nano-Co caused HIF-1α accumulation in the nucleus. In addition, hypoxia inducible factor 1α knock-out cells [HIF-1α (-/-)] and its wild-type cells [HIF-1α (+/+)] were used. Our results demonstrated that Nano-Co caused a dose- and time-dependent increase in Gadd45α expression in wild-type HIF-1α (+/+) cells, but only a slight increase in HIF-1α (-/-) cells. Pre-treatment of PW cells with heat shock protein 90 inhibitor, 17-(Allylamino)-17-demethoxygeldanamycin (17-AAG), prior to exposure to Nano-Co significantly abolished Nano-Co-induced Gadd45α expression. These results suggest that HIF-1α accumulation may be partially involved in the increased Gadd45α expression in cells exposed to Nano-Co. These findings may have important implications for understanding the potential health effects of metal nanoparticle exposure.
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