ArticleUltramicroscopy2014
Correlated cryo-fluorescence and cryo-electron microscopy with high spatial precision and improved sensitivity.
Article in Ultramicroscopy, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 65 papers.
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Who cites it
65 citing papers in PubMed.
- Integrated fluorescence light microscopy-guided cryo-focused ion beam-milling for in situ montage cryo-ET.Nature protocols · 2026Review
- Cryo-FIB-enabled 3D imaging of biological specimens.Applied microscopy · 2026Review
- Memory engram synapse 3D macromolecular architecture visualized by cryoCLEM-guided cryoET.Structure (London, England : 1993) · 2026Article
- Article
- Time-Resolved Fluorescence Imaging and Correlative Cryo-Electron Tomography to Study Structural Changes of the HIV-1 Capsid.ACS nano · 2025Article
- Article
- Memory engram synapse 3D molecular architecture visualized by cryoCLEM-guided cryoET.bioRxiv : the preprint server for biology · 2025Article
- StableMARK-decorated microtubules in cells have expanded lattices.The Journal of cell biology · 2025Article
- Recent advances in correlative cryo-light and electron microscopy.Current opinion in structural biology · 2024Review
- Article
- Centromeric chromatin clearings demarcate the site of kinetochore formation.bioRxiv : the preprint server for biology · 2024Article
- Stepwise assembly and release of Tc toxins from Yersinia entomophaga.Nature microbiology · 2024Article
- Insights into protein structure using cryogenic light microscopy.Biochemical Society transactions · 2023Review
- Correlative Light and Electron Cryo-Microscopy Workflow Combining Micropatterning, Ice Shield, and an In-Chamber Fluorescence Light Microscope.Bio-protocol · 2023Article
- Charting the molecular landscape of the cell.Structure (London, England : 1993) · 2023Review
- The in-tissue molecular architecture of β-amyloid pathology in the mammalian brain.Nature communications · 2023Article
- Bringing Structure to Cell Biology with Cryo-Electron Tomography.Annual review of biophysics · 2023Review
- HOPE-SIM, a cryo-structured illumination fluorescence microscopy system for accurately targeted cryo-electron tomography.Communications biology · 2023Article
- Cryo-electron tomography on focused ion beam lamellae transforms structural cell biology.Nature methods · 2023Review
- Integrated multimodality microscope for accurate and efficient target-guided cryo-lamellae preparation.Nature methods · 2023Article
5 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Performing fluorescence microscopy and electron microscopy on the same sample allows fluorescent signals to be used to identify and locate features of interest for subsequent imaging by electron microscopy. To carry out such correlative microscopy on vitrified samples appropriate for structural cryo-electron microscopy it is necessary to perform fluorescence microscopy at liquid-nitrogen temperatures. Here we describe an adaptation of a cryo-light microscopy stage to permit use of high-numerical aperture objectives. This allows high-sensitivity and high-resolution fluorescence microscopy of vitrified samples. We describe and apply a correlative cryo-fluorescence and cryo-electron microscopy workflow together with a fiducial bead-based image correlation procedure. This procedure allows us to locate fluorescent bacteriophages in cryo-electron microscopy images with an accuracy on the order of 50 nm, based on their fluorescent signal. It will allow the user to precisely and unambiguously identify and locate objects and events for subsequent high-resolution structural study, based on fluorescent signals.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.