ArticleCell cycle (Georgetown, Tex.)2014
Mitotic phosphorylation of histone H3 threonine 80.
Article in Cell cycle (Georgetown, Tex.), 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 33 citations in OpenAlex.
- Beyond the Usual Suspects: Examining the Role of Understudied Histone Variants in Breast Cancer.International journal of molecular sciences · 2024Review
- The 80Journal of microbiology and biotechnology · 2024Article
- Histone modification in podocyte injury of diabetic nephropathy.Journal of molecular medicine (Berlin, Germany) · 2022Review
- Dissecting the roles of Haspin and VRK1 in histone H3 phosphorylation during mitosis.Scientific reports · 2022Article
- The dark side of histones: genomic organization and role of oncohistones in cancer.Clinical epigenetics · 2021Review
- Priming chromatin for segregation: functional roles of mitotic histone modifications.Cell cycle (Georgetown, Tex.) · 2020Review
- Article
- Phosphorylation of Histone H4T80 Triggers DNA Damage Checkpoint Recovery.Molecular cell · 2018Article
- Baicalin hydrate inhibits cancer progression in nasopharyngeal carcinoma by affecting genome instability and splicing.Oncotarget · 2018Article
- Excess free histone H3 localizes to centrosomes for proteasome-mediated degradation during mitosis in metazoans.Cell cycle (Georgetown, Tex.) · 2016Article
- Preferential Phosphorylation on Old Histones during Early Mitosis in Human Cells.The Journal of biological chemistry · 2016Article
- Conservation and divergence of the histone code in nucleomorphs.Biology direct · 2016Article
- Mutations that prevent or mimic persistent post-translational modifications of the histone H3 globular domain cause lethality and growth defects in Drosophila.Epigenetics & chromatin · 2016Article
- H3 Thr3 phosphorylation is crucial for meiotic resumption and anaphase onset in oocyte meiosis.Cell cycle (Georgetown, Tex.) · 2016Article
- Mutation of histone H3 serine 86 disrupts GATA factor Ams2 expression and precise chromosome segregation in fission yeast.Scientific reports · 2015Article
- Comprehensive Catalog of Currently Documented Histone Modifications.Cold Spring Harbor perspectives in biology · 2015Review
- High phosphohistone H3 expression correlates with adverse clinical, biological, and pathological factors in neuroblastomas.The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society · 2015Article
- Quantitative proteomic analysis of histone modifications.Chemical reviews · 2015Review
- Emerging clinical applications of selected biomarkers in melanoma.Clinical, cosmetic and investigational dermatology · 2015Review
- Histone H3 Ser57 and Thr58 phosphorylation in the brain of 5XFAD mice.FEBS open bio · 2015Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
Abstract
The onset and regulation of mitosis is dependent on phosphorylation of a wide array of proteins. Among the proteins that are phosphorylated during mitosis is histone H3, which is heavily phosphorylated on its N-terminal tail. In addition, large-scale mass spectrometry screens have revealed that histone H3 phosphorylation can occur at multiple sites within its globular domain, yet detailed analyses of the functions of these phosphorylations are lacking. Here, we explore one such histone H3 phosphorylation site, threonine 80 (H3T80), which is located on the nucleosome surface. Phosphorylated H3T80 (H3T80ph) is enriched in metazoan cells undergoing mitosis. Unlike H3S10 and H3S28, H3T80 is not phosphorylated by the Aurora B kinase. Further, mutations of T80 to either glutamic acid, a phosphomimetic, or to alanine, an unmodifiable residue, result in an increase in cells in prophase and an increase in anaphase/telophase bridges, respectively. SILAC-coupled mass spectrometry shows that phosphorylated H3T80 (H3T80ph) preferentially interacts with histones H2A and H4 relative to non-phosphorylated H3T80, and this result is supported by increased binding of H3T80ph to histone octamers in vitro. These findings support a model where H3T80ph, protruding from the nucleosome surface, promotes interactions between adjacent nucleosomes to promote chromatin compaction during mitosis in metazoan cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.