ArticleEndocrinology2014
Role of cortactin in dynamic actin remodeling events in gonadotrope cells.
Article in Endocrinology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
16 citing papers in PubMed, 18 citations in OpenAlex.
- GnRH Induces Citrullination of the Cytoskeleton in Murine Gonadotrope Cells.International journal of molecular sciences · 2024Article
- The Hippo Pathway Effectors YAP and TAZ Regulate LH Release by Pituitary Gonadotrope Cells in Mice.Endocrinology · 2022Article
- Autophagy promotes invadopodia formation in human ovarian cancer cells via the p62-extracellular signal-regulated kinase 1/2 pathway.Experimental and therapeutic medicine · 2021Article
- Long extensions with varicosity-like structures in gonadotrope Lh cells facilitate clustering in medaka pituitary culture.PloS one · 2021Article
- Article
- Plasticity of Anterior Pituitary Gonadotrope Cells Facilitates the Pre-Ovulatory LH Surge.Frontiers in endocrinology · 2020Review
- A single tyrosine phosphorylation site in cortactin is important for filopodia formation in neuronal growth cones.Molecular biology of the cell · 2019Article
- Amastigote Synapse: The Tricks ofFrontiers in microbiology · 2018Article
- Lamin A/C Is Required for ChAT-Dependent Neuroblastoma Differentiation.Molecular neurobiology · 2017Article
- Gonadotropin releasing hormone activation of the mTORC2/Rictor complex regulates actin remodeling and ERK activity in LβT2 cells.Molecular and cellular endocrinology · 2017Article
- GnRH Induces ERK-Dependent Bleb Formation in Gonadotrope Cells, Involving Recruitment of Members of a GnRH Receptor-Associated Signalosome to the Blebs.Frontiers in endocrinology · 2017Article
- Functional Role of Gonadotrope Plasticity and Network Organization.Frontiers in endocrinology · 2017Review
- The F0F1 ATP Synthase Complex Localizes to Membrane Rafts in Gonadotrope Cells.Molecular endocrinology (Baltimore, Md.) · 2016Article
- Unique behavior of Trypanosoma cruzi mevalonate kinase: A conserved glycosomal enzyme involved in host cell invasion and signaling.Scientific reports · 2016Article
- Dynamin Is Required for GnRH Signaling to L-Type Calcium Channels and Activation of ERK.Endocrinology · 2016Article
- GnRH evokes localized subplasmalemmal calcium signaling in gonadotropes.Molecular endocrinology (Baltimore, Md.) · 2014Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
Abstract
GnRH induces marked activation of the actin cytoskeleton in gonadotropes; however, the physiological consequences and cellular mechanisms responsible have yet to be fully elucidated. The current studies focus on the actin scaffolding protein cortactin. Using the gonadotrope-derived αT3-1 cell line, we found that cortactin is phosphorylated at Y(421), S(405), and S(418) in a time-dependent manner in response to the GnRH agonist buserelin (GnRHa). GnRHa induced translocation of cortactin to the leading edge of the plasma membrane where it colocalizes with actin and actin-related protein 3 (Arp3). Incubation of αT3-1 cells with the c-src inhibitor phosphoprotein phosphatase 1, blocked tyrosine phosphorylation of cortactin, reduced cortactin association with Arp3, and blunted actin reorganization in response to GnRHa. Additionally, we used RNA silencing strategies to knock down cortactin in αT3-1 cells. Knockdown of cortactin blocked the ability of αT3-1 cells to generate filopodia, lamellipodia, and membrane ruffles in response to GnRHa. We show that lamellipodia and filopodia are capable of LHβ mobilization in primary pituitary culture after GnRHa treatment, and disruption of these structures using jasplakinolide reduces LH secretion. Collectively, our findings suggest that after GnRHa activation, src activity leads to tyrosine phosphorylation of cortactin, which facilitates its association with Arp3 to engage the actin cytoskeleton. The reorganization of actin by cortactin potentially underlies GnRHa-induced secretory events within αT3-1 cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.