ArticleThe Histochemical journal1986
Aminoalkylsilane-treated glass slides as support for in situ hybridization of keratin cDNAs to frozen tissue sections under varying fixation and pretreatment conditions.
Article in The Histochemical journal, 1986. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 90 papers.
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90 citing papers in PubMed, 381 citations in OpenAlex.
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- Expression of fibroblast growth factor-2 (FGF-2) in early stages (days 3-11) of the development of the avian lung, Gallus gallus variant domesticus: an immunocytochemical study.Journal of anatomy · 2003Article
- Spatiotemporal features of early neuronogenesis differ in wild-type and albino mouse retina.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2002Article
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- Lack of a phenotype in transgenic mice aberrantly expressing COL2A1 mRNA because of highly selective post-transcriptional down-regulation.The Biochemical journal · 2000Article
- Alteration of the leptin network in late morbid obesity induced in mice by brain infection with canine distemper virus.Journal of virology · 1999Article
- Inhibition of angiogenesis induces chromaffin differentiation and apoptosis in neuroblastoma.The American journal of pathology · 1999Article
- Polycystic kidney disease in SBM transgenic mice: role of c-myc in disease induction and progression.The American journal of pathology · 1998Article
- Role of neutrophils and mast cells in acute indomethacin-induced small bowel injury in the rat.Journal of gastroenterology · 1997Article
- Synchrony of clonal cell proliferation and contiguity of clonally related cells: production of mosaicism in the ventricular zone of developing mouse neocortex.The Journal of neuroscience : the official journal of the Society for Neuroscience · 1997Article
- VEGF and bFGF mRNA are expressed in ethylnitrosourea-induced experimental rat gliomas.Cellular and molecular neurobiology · 1997Article
- Hepatic protein synthesis in the regenerating rat liver after hepatopancreatectomy.Surgery today · 1997Article
- Widespread neuronal expression of the presenilin-1 early-onset Alzheimer's disease gene in the murine brain.The American journal of pathology · 1996Article
30 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Attempts to investigate the cellular localization of keratin mRNAs by in situ hybridization with specific [35S]-labelled cDNA probes to mouse epithelia have been seriously impeded by the uncontrollable detachment of frozen tissue sections on conventionally coated glass slides (i.e. those coated with egg white, gelatin, collagen). Similarly, a variety of other coating and attachment devices have proved to be unsatisfactory or impracticable for large scale investigations. These difficulties were completely overcome and in situ hybridization was possible after a short immersion of the glass slides in a 2% solution of 3-aminopropyltriethoxysilane in acetone. This treatment provides the glass surface with aminoalkyl groups which are apparently able to react covalently with aldehyde or ketone functions of frozen tissue sections. The resulting firm adhesion of the sections enabled us to investigate the influence of different fixation and prehybridization procedures on the quality of the in situ hybridization. It was found that especially harsh prehybridization, involving hydrochloric acid, heat and proteinase K treatment, drastically reduces the morphological integrity of the sections, thus rendering a reliable assignment of the label difficult. In contrast, a mild prehybridization, consisting mainly of a rehydration of the sections in phosphate-buffered saline and equilibration in 0.1 M glycine, leaves the morphology intact and leads to a highly efficient and specific in situ hybridization.
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