ArticleAnalytical chemistry2013
Isotopic ratio outlier analysis global metabolomics of Caenorhabditis elegans.
Article in Analytical chemistry, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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Who cites it
37 citing papers in PubMed, 56 citations in OpenAlex.
- Achieving Effective Batch-to-Batch Error Correction through Suppression Correction and Dual MSTUS Normalization.Analytical chemistry · 2026Article
- Ion suppression correction and normalization for non-targeted metabolomics.Nature communications · 2025Article
- Isotopic Ratio Outlier Analysis (IROA) for Quantitative Analysis.Methods in molecular biology (Clifton, N.J.) · 2025Article
- COiScience · 2023Article
- Mass Spectrometry-Based Multi-omics Integration with a Single Set ofAnalytical chemistry · 2023Article
- Isotope Ratio Outlier Analysis (IROA) for HPLC-TOFMS-Based Metabolomics of Human Urine.Metabolites · 2022Article
- Combining Isotopologue Workflows and Simultaneous Multidimensional Separations to Detect, Identify, and Validate Metabolites in Untargeted Analyses.Analytical chemistry · 2022Article
- Targeting unique biological signals on the fly to improve MS/MS coverage and identification efficiency in metabolomics.Analytica chimica acta · 2021Article
- Multi-omics insights into neuronal regeneration and re-innervation.Neural regeneration research · 2021Article
- Review
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- Enhanced Metabolome Coverage and Evaluation of Matrix Effects by the Use of Experimental-Condition-MatchedMetabolites · 2020Article
- Chemical Discovery in the Era of Metabolomics.Journal of the American Chemical Society · 2020Review
- Differentiation of soluble aqueous humor metabolites in primary open angle glaucoma and controls.Experimental eye research · 2020Article
- A Novel Cyanobacterium Synechococcus elongatus PCC 11802 has Distinct Genomic and Metabolomic Characteristics Compared to its Neighbor PCC 11801.Scientific reports · 2020Article
- Metabolic engineering of a fast-growing cyanobacteriumBiotechnology for biofuels · 2020Article
- Metabolomics of primary cutaneous melanoma and matched adjacent extratumoral microenvironment.PloS one · 2020Article
- An Isotopic Ratio Outlier Analysis Approach for Global Metabolomics of Biosynthetically Talented Actinomycetes.Metabolites · 2019Article
- Article
- Comparative physiological and metabolomics analysis of wheat (Triticum aestivum L.) following post-anthesis heat stress.PloS one · 2018Article
Corrections and comments
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Authors and funding
9 authors at 2 institutions in 1 country.
Funding
Abstract
We demonstrate the global metabolic analysis of Caenorhabditis elegans stress responses using a mass-spectrometry-based technique called isotopic ratio outlier analysis (IROA). In an IROA protocol, control and experimental samples are isotopically labeled with 95 and 5% (13)C, and the two sample populations are mixed together for uniform extraction, sample preparation, and LC-MS analysis. This labeling strategy provides several advantages over conventional approaches: (1) compounds arising from biosynthesis are easily distinguished from artifacts, (2) errors from sample extraction and preparation are minimized because the control and experiment are combined into a single sample, (3) measurement of both the molecular weight and the exact number of carbon atoms in each molecule provides extremely accurate molecular formulas, and (4) relative concentrations of all metabolites are easily determined. A heat-shock perturbation was conducted on C. elegans to demonstrate this approach. We identified many compounds that significantly changed upon heat shock, including several from the purine metabolism pathway. The metabolomic response information by IROA may be interpreted in the context of a wealth of genetic and proteomic information available for C. elegans . Furthermore, the IROA protocol can be applied to any organism that can be isotopically labeled, making it a powerful new tool in a global metabolomics pipeline.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.