ArticleProteins2014
Murine norovirus protein NS1/2 aspartate to glutamate mutation, sufficient for persistence, reorients side chain of surface exposed tryptophan within a novel structured domain.
Article in Proteins, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 19 citations in OpenAlex.
- Itaconate modulates immune responses via inhibition of peroxiredoxin 5.Nature metabolism · 2025Article
- Norovirus co-opts NINJ1 for selective protein secretion.Science advances · 2025Article
- The Disorderly Nature of Caliciviruses.Viruses · 2024Review
- Interferon responses to norovirus infections: current and future perspectives.The Journal of general virology · 2021Review
- Review
- Isolation and Identification of a Murine Norovirus Persistent Infection Strain in China.Frontiers in veterinary science · 2020Article
- Norovirus encounters in the gut: multifaceted interactions and disease outcomes.Mucosal immunology · 2019Review
- Recovirus NS1-2 Has Viroporin Activity That Induces Aberrant Cellular Calcium Signaling To Facilitate Virus Replication.mSphere · 2019Article
- Caspase-mediated cleavage of murine norovirus NS1/2 potentiates apoptosis and is required for persistent infection of intestinal epithelial cells.PLoS pathogens · 2019Article
- The Role of Interferon in Persistent Viral Infection: Insights from Murine Norovirus.Trends in microbiology · 2018Review
- Article
- Recent advances in understanding norovirus pathogenesis.Journal of medical virology · 2016Review
- A Review of Functional Motifs Utilized by Viruses.Proteomes · 2016Review
- Effects of murine norovirus on atherosclerosis in ldlr(-/-) mice depends on the timing of infection.Comparative medicine · 2015Article
- Interferon-λ cures persistent murine norovirus infection in the absence of adaptive immunity.Science (New York, N.Y.) · 2015Article
- Norovirus: targets and tools in antiviral drug discovery.Biochemical pharmacology · 2014Review
- Advances in norovirus biology.Cell host & microbe · 2014Review
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
Compact viral genomes such as those found in noroviruses, which cause significant enteric disease in humans, often encode only a few proteins, but affect a wide range of processes in their hosts and ensure efficient propagation of the virus. Both human and mouse noroviruses (MNVs) persistently replicate and are shed in stool, a highly effective strategy for spreading between hosts. For MNV, the presence of a glutamate rather than an aspartate at position 94 of the NS1/2 protein was previously shown to be essential for persistent replication and shedding. Here, we analyze these critical sequences of NS1/2 at the structural level. Using solution nuclear magnetic resonance methods, we determined folded NS1/2 domain structures from a nonpersistent murine norovirus strain CW3, a persistent strain CR6, and a persistent mutant strain CW3(D94E). We found an unstructured PEST-like domain followed by a novel folded domain in the N-terminus of NS1/2. All three forms of the domain are stable and monomeric in solution. Residue 94, critical for determining persistence, is located in a reverse turn following an α-helix in the folded domain. The longer side chain of glutamate, but not aspartate, allows interaction with the indole group of the nearby tryptophan, reshaping the surface of the domain. The discrimination between glutamyl and aspartyl residue is imposed by the stable tertiary conformation. These structural requirements correlate with the in vivo function of NS1/2 in persistence, a key element of norovirus biology and infection.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.