ArticleJournal of experimental botany2014
DER containing two consecutive GTP-binding domains plays an essential role in chloroplast ribosomal RNA processing and ribosome biogenesis in higher plants.
Article in Journal of experimental botany, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 28 citations in OpenAlex.
- The uS10c-BPG2 module mediates ribosomal RNA processing in chloroplast nucleoids.Nucleic acids research · 2024Article
- Article
- Plant organellar RNA maturation.The Plant cell · 2023Review
- The ribosome assembly GTPase EngA is involved in redox signaling in cyanobacteria.Frontiers in microbiology · 2023Article
- Regulatory Connections Between the Cyanobacterial Factor PipX and the Ribosome Assembly GTPase EngA.Frontiers in microbiology · 2021Article
- Glyceroglycolipid Metabolism Regulations under Phosphate Starvation Revealed by Transcriptome Analysis inMarine drugs · 2020Article
- Distinctive Features of PipX, a Unique Signaling Protein of Cyanobacteria.Life (Basel, Switzerland) · 2020Review
- The Photosystem II Repair Cycle Requires FtsH Turnover through the EngA GTPase.Plant physiology · 2018Article
- The ERA-Related GTPase AtERG2 Associated with Mitochondria 18S RNA Is Essential for Early Embryo Development inFrontiers in plant science · 2018Article
- Functional characterization of chloroplast-targeted RbgA GTPase in higher plants.Plant molecular biology · 2017Article
- Heterologous Expression of Der Homologs in an Escherichia coli der Mutant and Their Functional Complementation.Journal of bacteriology · 2016Article
- The nucleoid as a site of rRNA processing and ribosome assembly.Frontiers in plant science · 2014Article
- Establishment of the chloroplast genetic system in rice during early leaf development and at low temperatures.Frontiers in plant science · 2014Review
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Authors and funding
8 authors at 5 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
This study investigated protein characteristics and physiological functions of DER (Double Era-like GTPase) of higher plants. Nicotiana benthamiana DER (NbDER) contained two tandemly repeated GTP-binding domains (GD) and a C-terminal domain (CTD) that was similar to the K-homology domain involved in RNA binding. Both GDs possessed GTPase activity and contributed to the maximum GTPase activity of NbDER. NbDER fused to green fluorescent protein was localized primarily to chloroplast nucleoids. Arabidopsis der null mutants exhibited an embryonic lethal phenotype, indicating an essential function of DER during plant embryogenesis. Virus-induced gene silencing of NbDER resulted in a leaf-yellowing phenotype caused by disrupted chloroplast biogenesis. NbDER was associated primarily with the chloroplast 50S ribosomal subunit in vivo, and both the CTD and the two GD contributed to the association. Recombinant proteins of NbDER and its CTD could bind to 23S and 16S ribosomal RNAs in vitro. Depletion of NbDER impaired processing of plastid-encoded ribosomal RNAs, resulting in accumulation of the precursor rRNAs in the chloroplasts. NbDER-deficient chloroplasts contained significantly reduced levels of mature 23S and 16S rRNAs and diverse mRNAs in the polysomal fractions, suggesting decreased translation in chloroplasts. These results suggest that DER is involved in chloroplast rRNA processing and ribosome biogenesis in higher plants.
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