ArticleThe Plant cell2013
MICROTUBULE-ASSOCIATED PROTEIN65 is essential for maintenance of phragmoplast bipolarity and formation of the cell plate in Physcomitrella patens.
Article in The Plant cell, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 58 citations in OpenAlex.
- A Rab/Kinesin-12/kinase module couples vesicle delivery and phragmoplast dynamics during plant cell cytokinesis.The EMBO journal · 2026Article
- Cellular dynamics and molecular signaling networks of plant cytokinesis.Molecules and cells · 2026Review
- Class II kinesin-12 facilitates cell plate formation by transporting cell plate materials in the phragmoplast.Nature plants · 2025Article
- CRISPR/Cas9 targeted genetic screening in Physcomitrella identifies novel cell division genes.Frontiers in plant science · 2025Article
- The Potential Role ofPlants (Basel, Switzerland) · 2024Article
- Arabidopsis α-Aurora kinase plays a role in cytokinesis through regulating MAP65-3 association with microtubules at phragmoplast midzone.Nature communications · 2024Article
- Kinesin-4 optimizes microtubule orientations for responsive tip growth guidance in moss.The Journal of cell biology · 2023Article
- Article
- Cell biology of primary cell wall synthesis in plants.The Plant cell · 2022Review
- The bryophytes Physcomitrium patens and Marchantia polymorpha as model systems for studying evolutionary cell and developmental biology in plants.The Plant cell · 2022Review
- How plants grow under gravity conditions besides 1 g: perspectives from hypergravity and space experiments that employ bryophytes as a model organism.Plant molecular biology · 2021Review
- Phytophthora infestans RXLR effector AVR1 disturbs the growth of Physcomitrium patens without affecting Sec5 localization.PloS one · 2021Article
- The MossThe Plant cell · 2020Review
- Kinesin-13 and Kinesin-8 Function during Cell Growth and Division in the MossThe Plant cell · 2020Article
- Plant Kinesin-12: Localization Heterogeneity and Functional Implications.International journal of molecular sciences · 2019Review
- Kinetochore protein depletion underlies cytokinesis failure and somatic polyploidization in the mosseLife · 2019Article
- Dual localized kinesin-12 POK2 plays multiple roles during cell division and interacts with MAP65-3.EMBO reports · 2018Article
- The KCH Kinesin Drives Nuclear Transport and Cytoskeletal Coalescence to Promote Tip Cell Growth inThe Plant cell · 2018Article
- Conditional genetic screen in Physcomitrella patens reveals a novel microtubule depolymerizing-end-tracking protein.PLoS genetics · 2018Article
- Phragmoplast microtubule dynamics - a game of zones.Journal of cell science · 2018Review
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The phragmoplast, a plant-specific apparatus that mediates cytokinesis, mainly consists of microtubules (MTs) arranged in a bipolar fashion, such that their plus ends interdigitate at the equator. Membrane vesicles are thought to move along the MTs toward the equator and fuse to form the cell plate. Although several genes required for phragmoplast MT organization have been identified, the mechanisms that maintain the bipolarity of phragmoplasts remain poorly understood. Here, we show that engaging phragmoplast MTs in a bipolar fashion in protonemal cells of the moss Physcomitrella patens requires the conserved MT cross-linking protein MICROTUBULE-ASSOCIATED PROTEIN65 (MAP65). Simultaneous knockdown of the three MAP65s expressed in those cells severely compromised MT interdigitation at the phragmoplast equator after anaphase onset, resulting in the collapse of the phragmoplast in telophase. Cytokinetic vesicles initially localized to the anaphase midzone as normal but failed to further accumulate in the next several minutes, although the bipolarity of the MT array was preserved. Our data indicate that the presence of bipolar MT arrays is insufficient for vesicle accumulation at the equator and further suggest that MAP65-mediated MT interdigitation is a prerequisite for maintenance of bipolarity of the phragmoplast and accumulation and/or fusion of cell plate-destined vesicles at the equatorial plane.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.