ArticleNucleic acids research2014
Multiple replication origins with diverse control mechanisms in Haloarcula hispanica.
Article in Nucleic acids research, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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18 citing papers in PubMed, 30 citations in OpenAlex.
- Article
- A virus-borne DNA damage signaling pathway controls the lysogeny-induction switch in a group of temperate pleolipoviruses.Nucleic acids research · 2023Article
- Reprogramming the endogenous type I CRISPR-Cas system for simultaneous gene regulation and editing inmLife · 2022Article
- G-Quadruplexes in the Archaea Domain.Biomolecules · 2020Article
- Article
- Origins of DNA replication.PLoS genetics · 2019Review
- Influence of Origin Recognition Complex Proteins on the Copy Numbers of Three Chromosomes in Haloferax volcanii.Journal of bacteriology · 2018Article
- Characterization of Copy Number Control of Two Haloferax volcanii Replication Origins Using Deletion Mutants and Haloarchaeal Artificial Chromosomes.Journal of bacteriology · 2018Article
- Diversity of DNA Replication in the Archaea.Genes · 2017Review
- Genome Replication inFrontiers in microbiology · 2017Article
- Activation of a dormant replication origin is essential for Haloferax mediterranei lacking the primary origins.Nature communications · 2015Article
- The complete genome sequence and emendation of the hyperthermophilic, obligate iron-reducing archaeon "Geoglobus ahangari" strain 234(T).Standards in genomic sciences · 2015Article
- Choosing a suitable method for the identification of replication origins in microbial genomes.Frontiers in microbiology · 2015Review
- Association between the dynamics of multiple replication origins and the evolution of multireplicon genome architecture in haloarchaea.Genome biology and evolution · 2014Article
- DNA replication origins in archaea.Frontiers in microbiology · 2014Review
- Archaeal chromosome biology.Journal of molecular microbiology and biotechnology · 2014Review
- Diversity of the DNA replication system in the Archaea domain.Archaea (Vancouver, B.C.) · 2014Review
- Ori-Finder 2, an integrated tool to predict replication origins in the archaeal genomes.Frontiers in microbiology · 2014Article
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The use of multiple replication origins in archaea is not well understood. In particular, little is known about their specific control mechanisms. Here, we investigated the active replication origins in the three replicons of a halophilic archaeon, Haloarcula hispanica, by extensive gene deletion, DNA mutation and genome-wide marker frequency analyses. We revealed that individual origins are specifically dependent on their co-located cdc6 genes, and a single active origin/cdc6 pairing is essential and sufficient for each replicon. Notably, we demonstrated that the activities of oriC1 and oriC2, the two origins on the main chromosome, are differently controlled. A G-rich inverted repeat located in the internal region between the two inverted origin recognition boxes (ORBs) plays as an enhancer for oriC1, whereas the replication initiation at oriC2 is negatively regulated by an ORB-rich region located downstream of oriC2-cdc6E, likely via Cdc6E-titrating. The oriC2 placed on a plasmid is incompatible with the wild-type (but not the ΔoriC2) host strain, further indicating that strict control of the oriC2 activity is important for the cell. This is the first report revealing diverse control mechanisms of origins in haloarchaea, which has provided novel insights into the use and coordination of multiple replication origins in the domain of Archaea.
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